safety and t cell modulating effects of high dose vitamin d3 supplementation in multiple sclerosis安全性和t细胞调节的影响高剂量补充维生素d3在多发性硬化症.pdfVIP

Safety and T Cell Modulating Effects of High Dose Vitamin D3 Supplementation in Multiple Sclerosis 1,2,3 1,2,3 ¨ 2 1,2,5 Joost Smolders *, Evelyn Peelen , Marielle Thewissen , Jan Willem Cohen Tervaert , Paul Menheere4, Raymond Hupperts1,3, Jan Damoiseaux2,5 1 School for Mental Health and Neuroscience, Maastricht University Medical Center, Maastricht, The Netherlands, 2 Division of Clinical and Experimental Immunology, Department of Internal Medicine, Maastricht University Medical Center, Maastricht, The Netherlands, 3 Academic MS Center Limburg, Orbis Medical Center, Sittard, The Netherlands, 4 Department of Clinical Chemistry, Maastricht University Medical Center, Maastricht, The Netherlands, 5 Laboratory for Clinical Immunology, Maastricht University Medical Center, Maastricht, The Netherlands Abstract Background: A poor vitamin D status has been associated with a high disease activity of multiple sclerosis (MS). Recently, we described associations between vitamin D status and peripheral T cell characteristics in relapsing remitting MS (RRMS) patients. In the present study, we studied the effects of high dose vitamin D3 supplementation on safety and T cell related outcome measures. Methodology/Principal Findings: Fifteen RRMS patients were supplemented with 20 000 IU/d vitamin D3 for 12 weeks. Vitamin D and calcium metabolism were carefully monitored, and T cell characteristics were studied by flowcytometry. All patients finished the protocol without side-effects, hypercalcaemia, or hypercalciuria. The median vitamin D status increased from 50 nmol/L (31–175) at week 0 to 380 nmol/L