sequence composition and gene content of the short arm of rye (secale cereale) chromosome 1组成和基因序列的内容短臂黑麦(secale cereale)染色体1.pdfVIP

Sequence Composition and Gene Content of the Short Arm of Rye (Secale cereale) Chromosome 1 1 1 1 ˇ ´ 2 3 3 Silvia Fluch , Dieter Kopecky , Kornel Burg *, Hana Simkova , Stefan Taudien , Andreas Petzold , Marie ´ ´ 2 3 1 1 ˇ 2 4 Kubalakova , Matthias Platzer , Maria Berenyi , Siegfried Krainer , Jaroslav Dolezel , Tamas Lelley ´ 1 Health and Environment Department, Bioresources, Austrian Institute of Technology (AIT), Tulln, Austria, 2 Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic, 3 Leibniz Institute for Age Research (Fritz Lipmann Institute), Jena, Germany, 4 Department of Agrobiotechnology, Institute for Biotechnology in Plant Production (IFA-Tulln), University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria Abstract Background: The purpose of the study is to elucidate the sequence composition of the short arm of rye chromosome 1 (Secale cereale) with special focus on its gene content, because this portion of the rye genome is an integrated part of several hundreds of bread wheat varieties worldwide. Methodology/Principal Findings: Multiple Displacement Amplification of 1RS DNA, obtained from flow sorted 1RS chromosomes, using 1RS ditelosomic wheat-rye addition line, and subsequent Roche 454FLX sequencing o