sequence motifs in mads transcription factors responsible for specificity and diversification of protein-protein interaction序列图案在尼负责特异性转录因子和多样化的蛋白质交互.pdfVIP

Sequence Motifs in MADS Transcription Factors Responsible for Specificity and Diversification of Protein- Protein Interaction 1 2¤ 1 1 Aalt D. J. van Dijk , Giuseppa Morabito , Martijn Fiers , Roeland C. H. J. van Ham , Gerco C. Angenent1,2, Richard G. H. Immink1,2* 1 Plant Research International, Bioscience, Wageningen, The Netherlands, 2 Centre for BioSystems Genomics (CBSG), Wageningen, The Netherlands Abstract Protein sequences encompass tertiary structures and contain information about specific molecular interactions, which in turn determine biological functions of proteins. Knowledge about how protein sequences define interaction specificity is largely missing, in particular for paralogous protein families with high sequence similarity, such as the plant MADS domain transcription factor family. In comparison to the situation in mammalian species, this important family of transcription regulators has expanded enormously in plant species and contains over 100 members in the model plant species Arabidopsis thaliana. Here, we provide insight into the mechanisms that determine protein-protein interaction specificity for the Arabidopsis MADS domain transcription factor family, using an integrated computational and experimental approach. Plant MADS proteins have highly similar amino acid sequences, but their dimerization patterns vary substantially. Our computational analysis uncovered small sequence regions that explain observed differences in dimerization patterns with reasonable accuracy. Furthermore, we show the usefulness of the method for prediction of MADS domain transcription factor interaction networks in other plant species. Intr