原创力文档simple field assays to check quality of current artemisinin-based antimalarial combination formulations简单的字段化验检查当前青蒿素为基础的抗疟组合配方的质量.pdfVIP
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simple field assays to check quality of current artemisinin-based antimalarial combination formulations简单的字段化验检查当前青蒿素为基础的抗疟组合配方的质量
Simple Field Assays to Check Quality of Current
Artemisinin-Based Antimalarial Combination
Formulations
Jean-Robert Ioset¤a, Harparkash Kaur*
London School of Hygiene and Tropical Medicine, London, United Kingdom
Abstract
Introduction: Malaria continues to be one of the major public health problems in Africa, Asia and Latin America. Artemisinin
derivatives (ARTs; artesunate, artemether, and dihydroartemisinin) derived from the herb, Artemisia annua, are the most
effective antimalarial drugs available providing rapid cures. The World Health Organisation (WHO) has recommended that
all antimalarials must be combined with an artemisinin component (artemisinin-based combination therapy; ACT) for use as
first line treatment against malaria. This class of drugs is now first-line policy in most malaria-endemic countries. Reports of
ad hoc surveys from South East Asia show that up to 50% of the artesunate currently sold is counterfeit. Drug quality is
rarely assessed in resource poor countries in part due to lack of dedicated laboratory facilities which are expensive to build,
equip and maintain. With a view to address this unmet need we developed two novel colour reaction assays that can be
used in the field to check the quality of ARTs.
Methods and Findings: Our assays utilise thin layer chromatography silica gel sheets and 2, 4 dinitrophenylhydrazine or 4-
Benzoylamino-2, 5-dimethoxybenzenediazonium chloride hemi (zinc chloride) salt as the reagents showing a pink or blue
product respectively only in the presence ARTs. We are able to detect as low as 10% of ARTs in ACTs (WINTHROP -
H
artesunate/amodiaquine, Coartem -artemether/lumefantrine and Duocortexcin - dihydroartemisinin/piperaquine). The
assays have been validated extensively by testing eighty r
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