sequencing and de novo analysis of crassostrea angulata (fujian oyster) from 8 different developing phases using 454 gsflx测序和新创的分析crassostrea angulata(福建牡蛎)使用454 gsflx 8种不同的发展阶段.pdfVIP

Sequencing and de novo Analysis of Crassostrea angulata (Fujian Oyster) from 8 Different Developing Phases Using 454 GSFlx 1,2. 1,2. 1,2 3 1,2 1,2 Ji Qin , Zixia Huang , Jun Chen , Quan Zou , Weiwei You , Caihuan Ke * 1 State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China, 2 College of Ocean and Earth Sciences, Xiamen University, Xiamen, China, 3 College of Information Science and Technology, Xiamen University, Xiamen, China Abstract Research on the mechanism for early development of shellfish, such as body plan, shell formation, settlement and metamorphosis is currently an active research field. However, studies were still limited and not deep enough because of the lack of genomic resources such as genome or transcriptome sequences. In the present research, de novo transcriptome sequencing was performed for Crassostrea angulata, the most economically important cultured oyster species in China, at eight early developmental stages using the 454 sequencing technology. A total of 555,215 reads were produced with an average length of 309 nucleotides that were then assembled into 10,462 contigs. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes recovered diverse biological functions and processes. Six unique sequences related to settlement, metamorphosis and growth were subsequently analyzed by real-time PCR. Given the lack of whole genome information for oysters, transcriptome and de novo analysis of C. angulata from the eight different developing phases will provide important and useful information on early development mechanism and help genetic breeding of shellfish. Citation: Qi