sequence-specific capture of protein-dna complexes for mass spectrometric protein identificationsequence-specific捕获蛋白质protein-dna复合物的质谱鉴定.pdfVIP

Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification 1,2 1 1 1 1 Cheng-Hsien Wu , Siyuan Chen , Michael R. Shortreed , Gloria M. Kreitinger , Yuan Yuan , Brian L. 1 3 3,4 5 3,6 1,2,7 Frey , Yi Zhang , Shama Mirza , Lisa A. Cirillo , Michael Olivier , Lloyd M. Smith * 1 Department of Chemistry, University of Wisconsin, Madison, Wisconsin, United States of America, 2 Program in Cellular and Molecular Biology, University of Wisconsin- Madison, Madison, Wisconsin, United States of America, 3 Biotechnology and Bioengineering Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 4 Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 5 Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 6 Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America, 7 Genome Center of Wisconsin, Madison, Wisconsin, United States of America Abstract The regulation of gene transcription is fundamental to the existence of complex multicellular organisms such as humans. Although it is widely recognized that much of gene regulation is controlled by gene-specific protein-DNA interactions, there presently exists little in the way of tools to identify proteins that interact with the genome at locations of interest. We have developed a novel strategy to address this problem, which we refer to as GENECAPP, for Global ExoNuclease-based E