screening of trypanosoma brucei gambiense in domestic livestock and tsetse flies from an insular endemic focus (luba, equatorial guinea)筛选锥虫属brucei gambiense家畜和采采蝇从一个孤立的流行焦点(班图语、赤道几内亚).pdfVIP

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screening of trypanosoma brucei gambiense in domestic livestock and tsetse flies from an insular endemic focus (luba, equatorial guinea)筛选锥虫属brucei gambiense家畜和采采蝇从一个孤立的流行焦点(班图语、赤道几内亚).pdf

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screening of trypanosoma brucei gambiense in domestic livestock and tsetse flies from an insular endemic focus (luba, equatorial guinea)筛选锥虫属brucei gambiense家畜和采采蝇从一个孤立的流行焦点(班图语、赤道几内亚)

Screening of Trypanosoma brucei gambiense in Domestic Livestock and Tsetse Flies from an Insular Endemic Focus (Luba, Equatorial Guinea) 1 ´ ´ 1 ´ 2,3 ´ 4 Carlos Cordon-Obras *, Carmen Garcıa-Estebanez , Nicolas Ndong-Mabale , Simon Abaga , Pedro 3 ´ 1 1,2 Ndongo-Asumu , Agustın Benito , Jorge Cano 1 National Centre of Tropical Medicine (Institute of Health Carlos III), Madrid, Spain, 2 Reference Centre for Endemic Diseases Control in Equatorial Guinea, Ministry of Health and Social Welfare, Malabo, Equatorial Guinea, 3 National Sleeping Sickness Control Programme, Ministry of Health and Social Welfare, Bata, Equatorial Guinea, 4 National Programme for Malaria Control, Ministry of Health and Social Welfare, Malabo, Equatorial Guinea Abstract Background: Sleeping sickness is spread over 36 Sub-Saharan African countries. In West and Central Africa, the disease is caused by Trypanosoma brucei gambiense, which produces a chronic clinical manifestation. The Luba focus (Bioko Island, Equatorial Guinea) has not reported autochthonous sleeping sickness cases since 1995, but given the complexity of the epidemiological cycle, the elimination of the parasite in the environment is difficult to categorically ensure. Methodology/Principal Findings: The aim of this work is to assess, by a molecular approach (Polymerase Chain Reaction, PCR), the possible permanence of T. b. gambiense in the vector (Glossina spp.) and domestic fauna in order to improve our understanding of the epidemiological situation of the disease in an isolated focus considered to be under control. The results obtained show the absence of the parasite in peridomestic livestock but