role of snx16 in the dynamics of tubulo-cisternal membrane domains of late endosomes角色snx16 tubulo-cisternal膜的动力学领域的核内体.pdfVIP

Role of SNX16 in the Dynamics of Tubulo-Cisternal Membrane Domains of Late Endosomes 1 ´ 1¤ 2 1 Ben Brankatschk , Veronique Pons , Robert G. Parton , Jean Gruenberg * 1 Department of Biochemistry, University of Geneva, Geneva, Switzerland, 2 Institute for Molecular Bioscience and Center for Microscopy and Microanalysis, University of Queensland, Brisbane, Australia Abstract In this paper, we report that the PX domain-containing protein SNX16, a member of the sorting nexin family, is associated with late endosome membranes. We find that SNX16 is selectively enriched on tubulo-cisternal elements of this membrane system, whose highly dynamic properties and formation depend on intact microtubules. By contrast, SNX16 was not found on vacuolar elements that typically contain LBPA, and thus presumably correspond to multivesicular endosomes. We conclude that SNX16, together with its partner phosphoinositide, define a highly dynamic subset of late endosomal membranes, supporting the notion that late endosomes are organized in distinct morphological and functional regions. Our data also indicate that SNX16 is involved in tubule formation and cholesterol transport as well as trafficking of the tetraspanin CD81, suggesting that the protein plays a role in the regulation of late endosome membrane dynamics. Citation: Brankatschk B, Pons V, Parton RG, Gruenberg J (2011) Role of SNX16 in the Dynamics of Tubulo-Cisternal Membrane Domains of Late Endosomes. PLoS ONE 6(7): e21771. doi:10.1371/journal.pone.0021771 Editor: Joshua Z. Rappoport, University of Birmingham, United Kingdom Received February 2, 2011; Accepted June 7, 2011; Published July 6, 2011 Copyright: 2011 Brankatschk et al. This is an open-access article distr