rickettsia species in african anopheles mosquitoes立克次氏体物种在非洲按蚊.pdfVIP

Rickettsia Species in African Anopheles Mosquitoes 1 ´ ´ 2 3 1 1 Cristina Socolovschi , Frederic Pages , Mamadou O. Ndiath , Pavel Ratmanov , Didier Raoult * ´ ´ ´ 1 Aix Marseille Universite, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France, 2 CIRE/ARS Ocean Indien, La Reunion, France, 3 IRD, Laboratoire de ´ ´ Paludologie, UMR 198 URMITE, Dakar, Senegal Abstract Background: There is higher rate of R. felis infection among febrile patients than in healthy people in Sub-Saharan Africa, predominantly in the rainy season. Mosquitoes possess a high vectorial capacity and, because of their abundance and aggressiveness, likely play a role in rickettsial epidemiology. Methodology/Principal Findings: Quantitative and traditional PCR assays specific for Rickettsia genes detected rickettsial ˆ DNA in 13 of 848 (1.5%) Anopheles mosquitoes collected from Cote d’Ivoire, Gabon, and Senegal. R. felis was detected in one ˆ An. gambiae molecular form S mosquito collected from Kahin, Cote d’Ivoire (1/77, 1.3%). Additionally, a new Rickettsia ˆ genotype was detected in five An. gambiae molecular form S mosquitoes collected from Cote d’Ivoire (5/77, 6.5%) and one mosquito from Libreville, Gabon (1/88, 1.1%), as well as six An.