role of peptidergic nerve terminals in the skin reversal of thermal sensation by calcitonin gene-related peptide in trpv1-depleted neuropathy皮肤作用的肽能的神经终端逆转热感觉的降钙素相关基因肽trpv1-depleted神经病变.pdfVIP

Role of Peptidergic Nerve Terminals in the Skin: Reversal of Thermal Sensation by Calcitonin Gene-Related Peptide in TRPV1-Depleted Neuropathy 1,2. 3,4. 2 5 2 Yu-Lin Hsieh , Chih-Lung Lin , Hao Chiang , Yaw-Syan Fu , June-Horng Lue , Sung- Tsang Hsieh2,6,7* 1 Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, 2 Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, 3 Department of Neurosurgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, 4 Faculty of Medicine, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, 5 Department of Biomedical Science and Environmental Biology, College of Science, Kaohsiung Medical University, Kaohsiung, Taiwan, 6 Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan, 7 Graduate Institute of Brain and Mind Sciences, College of Medicine, National Taiwan University, Taipei, Taiwan Abstract To investigate the contribution of peptidergic intraepidermal nerve fibers (IENFs) to nociceptive responses after depletion of the thermal-sensitive receptor, transient receptor potential vanilloid subtype 1 (TRPV1), we took advantage of a resiniferatoxin (RTX)-induced neuropathy which specifically affected small-diameter dorsal root ganglion (DRG) neurons and their corresponding nerve terminals in the skin. Thermal hypoalgesia (p ,0.001) developed from RTX-treatment day 7 (RTXd7) and became normalized from RTXd56 to RTXd84. Substance P (SP)(+) and TRPV1(+) neurons were completely depleted (p = 0.0001 and p ,0.0001, respectively), but RTX had a relatively minor effect on calcitonin gene-rela