spatio-temporal dynamics of yeast mitochondrial biogenesis transcriptional and post-transcriptional mrna oscillatory modules时空动态酵母线粒体生物起源mrna转录和转录后振荡模块.pdfVIP

Spatio-Temporal Dynamics of Yeast Mitochondrial Biogenesis: Transcriptional and Post-Transcriptional mRNA Oscillatory Modules ¨ 1 2 3 2 ` 4 Gaelle Lelandais *, Yann Saint-Georges , Colette Geneix , Liza Al-Shikhley , Genevieve Dujardin , Claude Jacq2* ´ ´ 1 Dynamique des Structures et Interactions des Macromolecules Biologiques (DSIMB), INSERM UMR-S 665, Universite Paris Diderot, Paris, France, 2 Laboratoire de ´ ´ ´ ´ ´ Genetique Moleculaire, CNRS UMR 8541, Ecole Normale Superieure, Paris, France, 3 MTI, INSERM UMR-S 973, Universite Paris Diderot, Paris, France, 4 Laboratoire de ´ ´ ´ Genetique Moleculaire, CNRS, Gif/Yvette, France Abstract Examples of metabolic rhythms have recently emerged from studies of budding yeast. High density microarray analyses have produced a remarkably detailed picture of cycling gene expression that could be clustered according to metabolic functions. We developed a model-based approach for the decomposition of expression to analyze these data and to identify functional modules which, expressed sequentially and periodically, contribute to the complex and intricate mitochondrial architecture. This approach revealed that mitochondrial spatio-temporal modules are expressed during periodic spikes and specific cellular localizations, which cover the entire oscillatory period. For instance, assembly factors (32 genes) and translation regulators (47 genes) are expressed earlier than the components of the amino-acid synthesis path