spatiotemporal correlations between blood-brain barrier permeability and apparent diffusion coefficient in a rat model of ischemic stroke时空的血脑屏障通透性和表观扩散系数之间的相关性在缺血性中风的大鼠模型.pdfVIP

Spatiotemporal Correlations between Blood-Brain Barrier Permeability and Apparent Diffusion Coefficient in a Rat Model of Ischemic Stroke Saeid Taheri., Eduardo Candelario-Jalil., Eduardo Y. Estrada, Gary A. Rosenberg* Department of Neurology, University of New Mexico, Health Sciences Center, Albuquerque, New Mexico, United States of America Abstract Variations in apparent diffusion coefficient of water (ADC) and blood-brain barrier (BBB) permeability after ischemia have been suggested, though the correlation between ADC alterations and BBB opening remains to be studied. We hypothesized that there are correlations between the alteration of ADC and BBB permeability. Rats were subjected to 2 h of transient middle cerebral artery occlusion and studied at 3 and 48 h of reperfusion, which are crucial times of BBB opening. BBB permeability and ADC values were measured by dynamic contrast-enhanced MRI and diffusion-weighted imaging, respectively. Temporal and spatial analyses of the evolution of BBB permeability and ADC alteration in cortical and subcortical regions were conducted along with the correlation between ADC and BBB permeability data. We found significant increases in BBB leakage and reduction in ADC values between 3 and 48 h of reperfusion. We identified three MR tissue signature models: high K and low ADC, high K and normal ADC, and normal K and low ADC. Over time, areas with i i i normal K and low ADC transformed into areas with high K . We observed a pattern of lesion evolution where the extent of i i initial ischemic injury reflect