spatio-temporal expression profile of stem cell-associated gene lgr5 in the intestine during thyroid hormone-dependent metamorphosis in xenopus laevis茎细胞相关基因的时空表达谱在小肠甲状腺激素依赖性蜕变lgr5就在非洲爪蟾蜍光滑的.pdfVIP

Spatio-Temporal Expression Profile of Stem Cell- Associated Gene LGR5 in the Intestine during Thyroid Hormone-Dependent Metamorphosis in Xenopus laevis 1. 2. 1. 1 1 1 Guihong Sun , Takashi Hasebe , Kenta Fujimoto , Rosemary Lu , Liezhen Fu , Hiroki Matsuda , 3 2,3 1 Mitsuko Kajita , Atsuko Ishizuya-Oka , Yun-Bo Shi * 1 Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, Program in Cellular Regulation and Metabolism (PCRM), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, Maryland, United States of America, 2 Department of Biology, Nippon Medical School, Kawasaki, Kanagawa, Japan, 3 Department of Molecular Biology, Institute of Development and Aging Sciences, Nippon Medical School, Kawasaki, Kanagawa, Japan Abstract Background: The intestinal epithelium undergoes constant self-renewal throughout adult life across vertebrates. This is accomplished through the proliferation and subsequent differentiation of the adult stem cells. This self-renewal system is established in the so-called postembryonic developmental period in mammals when endogenous thyroid hormone (T3) levels are high. Methodology/Principal Findings: The T3-dependent metamorphosis in anurans like Xenopus laevis resembles the mammalian postembryonic development and offers a unique opportunity to study how the adult stem cells are developed. The tadpole intestine is predominantly a monolayer of larval epithelial cells. During metamorphosis, the larval epithelial cells undergo apoptos