species difference of cd137 ligand signaling in human and murine monocytes物种的差异cd137配体在人类和小鼠单核细胞信号.pdfVIP

Species Difference of CD137 Ligand Signaling in Human and Murine Monocytes 1,2 1 1 ´ ´ 1 1,2 Qianqiao Tang , Dongsheng Jiang , Zhe Shao , Julia M. Martınez Gomez , Herbert Schwarz * 1 Department of Physiology, and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, 2 NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore Abstract Background: Stimulation of CD137 ligand on human monocytes has been shown to induce DC differentiation, and these CD137L-DCs are more potent than classical DCs, in stimulating T cell responses in vitro. To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs. Methodology/Principal Findings: When stimulated through CD137 ligand murine monocytes responded just as human monocytes with an increased adherence, morphological changes, proliferation and an increase in viable cell numbers. But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity. Murine monocytes did not differentiate to inflammatory DCs upon CD137 ligand signaling. Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs. Conclusions/Significance: These data demonstrate that both human and murine monocytes become activated by CD137 ligand signaling but only human and not murine monocytes differentiate to inflammatory DCs. ´ ´ Citation: Tang Q, Jiang D