single-species microarrays and comparative transcriptomics单一物种微阵列和比较转录组.pdfVIP

Single-Species Microarrays and Comparative Transcriptomics ´ ´ 1 2 1 Frederic J. J. Chain , Dora Ilieva , Ben J. Evans * 1 Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, Ontario, Canada, 2 Michael DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada Abstract Background: Prefabricated expression microarrays are currently available for only a few species but methods have been proposed to extend their application to comparisons between divergent genomes. Methodology/Principal Findings: Here we demonstrate that the hybridization intensity of genomic DNA is a poor basis on which to select unbiased probes on Affymetrix expression arrays for studies of comparative transcriptomics, and that doing so produces spurious results. We used the Affymetrix Xenopus laevis microarray to evaluate expression divergence between X. laevis, X. borealis, and their F1 hybrids. When data are analyzed with probes that interrogate only sequences with confirmed identity in both species, we recover results that differ substantially analyses that use genomic DNA hybridizations to select probes. Conclusions/Significance: Our findings have implications for the experimental design of comparative expression studies that use single-species microarrays, and for our understanding of divergent expression in hybrid clawed frogs. These findings also highlight important limitations of single-species microarrays for studies of comparative transcriptomics of polyploid species. Citation: Chain FJJ, Ilieva D, Evans BJ (2008) Single-Species Microarrays and Comparative Transcriptomics. PLoS ONE 3(9): e3279. doi:10.1371/ journal.pone.0003279 Editor: Dawn N. Albertson, Minnesota State University Mankato, United States of America