single molecule imaging reveals differences in microtubule track selection between kinesin motors单分子成像揭示了微管跟踪选择差异驱动蛋白马达.pdfVIP

Single Molecule Imaging Reveals Differences in Microtubule Track Selection Between Kinesin Motors 1,2 3 3 2,4 1,2 Dawen Cai , Dyke P. McEwen , Jeffery R. Martens , Edgar Meyhofer , Kristen J. Verhey * 1 Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America, 2 Biophysics Research Division, University of Michigan, Ann Arbor, Michigan, United States of America, 3 Department of Pharmacology, University of Michigan, Ann Arbor, Michigan, United States of America, 4 Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, United States of America Abstract Cells generate diverse microtubule populations by polymerization of a common a/ b-tubulin building block. How microtubule associated proteins translate microtubule heterogeneity into specific cellular functions is not clear. We evaluated the ability of kinesin motors involved in vesicle transport to read microtubule heterogeneity by using single molecule imaging in live cells. We show that individual Kinesin-1 motors move preferentially on a subset of microtubules in COS cells, identified as the stable microtubules marked by post-translational modifications. In contrast, individual Kinesin-2 (KIF17) and Kinesin-3 (KIF1A) motors do not select subsets of microtubules. Surprisingly, KIF17 and KIF1A motors that overtake the plus ends of growing microtubules do not fall off but rather track with the growing tip. Selection of microtubule tracks restricts Kinesin-1 transport of VSVG vesicles to s