serial non-invasive monitoring of renal disease following immune-mediated injury using near-infrared optical imaging串行非侵入性监测肾脏疾病免疫介导的损伤后使用近红外光学成像.pdfVIP

Serial Non-Invasive Monitoring of Renal Disease Following Immune-Mediated Injury Using Near-Infrared Optical Imaging 1 1 2 1 1 2 1 Yong Du , Shion An , Li Liu , Li Li , Xin J. Zhou , Ralph P. Mason *, Chandra Mohan * 1 Internal Medicine/Rheumatology, University of Texas, Southwestern Medical Center, Dallas, Texas, United States of America, 2 Radiology, University of Texas, Southwestern Medical Center, Dallas, Texas, United States of America Abstract Background: Non-invasive monitoring of disease progression in kidney disease is still a major challenge in clinical practice. In vivo near-infrared (NIR) imaging provides a new tool for studying disease mechanisms and non-invasive monitoring of disease development, even in deep organs. The LI-COR IRDyeH 800CW RGD optical probe (RGD probe) is a NIR fluorophore, that can target integrin alpha v beta 3 (a b ) in tissues. v 3 Objective: This study aims to monitor renal disease progression in an anti-glomerular basement membrane (GBM) nephritis mouse model. Methods: Anti-GBM nephritis was induced in 129x1/svJ mice by anti-GBM serum challenge. The expression of integrin a b v 3 in the diseased kidney was examined by immunohistochemistry and quantitative polymerase chain reaction. The RGD probe and control fluorophores, the 800CW dye, and the BSA-conjugated 800CW dye, were administered into anti-GBM nephritic mice. LI-COR PearlH Impulse imaging system was used for in vivo imaging; while ex vivo organ imaging was acquired using the MaestroTM imaging system. Results: Ki