微生物遗传育种21的.docVIP

1 Repair of thymine dimers is that the daughter strands have large gaps. one for each unexcised thymine dimer. There is on way to produce viable daughter cell by continued replication alone because the strands having the thymine dimer will continued to turn out gapped daughter strands, and the first set of gapped daughter strands will be fragmented when the enters a gap. By a recombination mechanism involving sister-strand exchange, however, an intact double-stranded molecule can be made The essential idea sister-strand exchange is that single-stranded segment free of any defects is excised from the homologous DNA segment and inserted into the gap produced opposite the thymine dimer (Figure 9-12).This genetic recombination event requires the RecA protein. DNA Pol I then synthesjzes the complementary strand, and DNA ligase joins this inserted piece to adjacent DNA, thus filling in the gap. The gap formed by excision of one strand from the donor is repaired by DNA Pol I and DNA ligase. If each thymine dimer is repaired this way, two complete daughter single strands can be formed, and each can serve in the next round of replication as a template for synposite strands are near one another because then no undamaged sister-strand segments are available to recombine with the other strand. Many molecular details of recombinational repair are still not known, so the model shown in Figure 9-12 is simply a model. Recombination repair is an important mechanism because it eliminates the necessity for delaying replication for the many hours that would be needed foe excision repair to remove all thymine dimers. Furthermore, recombination repair may correct some kinds of damage that cannot be corrected by excision repair-for example, alterations that do not cause be helix distortion but do stop DNA synthesis. Recombinational repair can also occur with UV-irradiated phages. If a population of a phage that fails to engage in excision repair is UV-irradiated, fewer phage pla