纯化方法.pptVIP

“ ” 纯化方法 报告人：候文思 实验试剂： Buffer： Buffer A: 100mM Tris-HCl,1mM EDTA,pH7.0 Buffer B: 100mM Tris-HCl,150mM NaCl,1.5% Triton X 100, pH 7.0 Buffer B*: 100mM Tris-HCl,150mM NaCl,0.5% Triton X 100, pH 7.0 Buffer B**: 100mM Tris-HCl,150mM NaCl,pH7.0 Buffer C: 6M GdnHCl, 100mM Tris-HCl,100mM DTT, 5mM EDTA, pH8.0 Buffer D: 4M GdnHCl, 100mM Na3PO4,pH6.0 Buffer D*: 4M GdnHCl, 100mM Na3PO4,1mM TCEP, pH8.0 (pH6.3,pH4.5) Buffer E: 1M L-arginine, 1mM EDTA, 0.05M Na3PO4, 5mM reduced GSH, 1mM oxidized GSSG, pH 8.0 Buffer F: 20mM Tris-HCl,1mM EDTA, 10% glycerol, 50mM NaCl,pH7.3 Buffer F*: 20mM Tris-HCl,1mM EDT