灵芝孢子油对鼠肝纤维化的干预作用.PDFVIP

灵芝孢子油对大鼠肝纤维化的干预作用  赵灏 1 ，胡芳红 2 ，李明焱 3 ，陈伟平 2*(1.杭州市西溪医院，杭州 310023 ；2.浙江大学城市学院，杭州 310015 ；3.金华寿仙谷药业 有限公司，浙江 金华 321200) 摘要：目的 探讨灵芝孢子油对大鼠肝纤维化的影响。方法 选择 40% CCl4 皮下注射诱导 SD 大鼠肝纤维化形成，造模 同时用低、中、高剂量(0.33 ，0.67 ，2.00 g·kg1)灵芝孢子油灌胃干预(每组 10 只) ，观察血浆谷草转氨酶(AST)、谷丙转氨 酶(ALT)、丙二醛(MDA)、透明质酸(HA)和肝组织 MDA 、羟脯氨酸(HyP)等的变化，肝组织 HE 和 Masson 染色观察病理 变化。结果 灵芝孢子油能显著降低 CCl4 所致的肝纤维化大鼠血浆 AST 、ALT 、MDA 、HA 水平(P0.05) ，降低肝组织 HyP 、MDA 含量(P0.05) ，肝脏病理显示纤维化程度明显减轻(P0.05) 。结论 灵芝孢子油对 CCl4 诱导的大鼠肝纤维化 具有保护作用。 关键词：肝纤维化；灵芝孢子油；四氯化碳；氧化损伤 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2016)10-1268-05 DOI: 10.13748/ki.issn1007-7693.2016.10.011 Antifibrotic Effect of Ganoderma Lucidum Spore Oil in Rats 1 2 3 2* ZHAO Hao , HU Fanghong , LI Mingyan , CHEN Weiping (1.Xixi Hospital of Hangzhou, Hangzhou 310023, China; 2.Zhejiang University City College, Hangzhou 310015, China; 3.Jinhua Shou Valley Pharmaceutical Co., Ltd., Jinhua 321200, China) ABSTRACT: OBJECTIVE To investigate the effect of Ganoderma lucidum spore oil on liver fibrosis. METHODS Liver fibrosis was replicated by 40% CCl4 injection subcutaneously in SD male rats. Meantime, three doses of Ganoderma lucidum spore oil low, medium and high(0.33, 0.67, 2.00 g·kg1) were used to intervene the formation of fibrosis by gavage (n=10, per group). Plasma activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) and hyaluronic acid (HA) , and the hepatic levels of MDA and hydroxyproline (HyP) were evaluated. Hematoxylin and eosin(HE) and Masson staining were used to observe the change of liver histology. RESULTS Plasma activities of AST, ALT, MDA and HA all were reduced significantly by Ganoderma lucidum spore oil in CCl4 induced rats (P0.05). Similar change was observed in hepatic content of Hyp and MDA(P0.05), and further conformed by