氢化可松对人A375黑素瘤细胞株黑素合成影响.pdfVIP

Effects of hydrocortisone on the synthesis of melanin in A375 human melanoma cells. Abstract Object: To observe the cell growth, melanogenesis and tyrosinase activity in A375 human melanoma cells after treatment with hydrocortisone of different concentration ;to study the mechanism of hydrocortisone in treating vitiligo. Methods : To treat A375 human melanoma cells in different concentration of hydrocortisone，to observe the growth of A375 human melanoma cells by MTT, to measure the content of melanin by spallation of NaOH, to measure the change of tyrosinase with dioxyphenylalanine oxidation. Results:1）When the concentration of hydrocortisone was between 10-7 g/L and 10-5g/L，it showed an increasing tendency of the amount of A375 human melanoma cells with the concentration of hydrocortisone raised, while the concentration of hydrocortisone was between 10-5 -1 and 10 g/L, it showed a decreasing tendency of the amount of A375 human melanoma cells with the concentration of hydrocortisone raised, compared with the control, when the concentration of hydrocortisone was at 10-5g/L , the cells proliferated obviously, the volume of the cells accreted; while he concentration of hydrocortisone was at 10-1g/L ,the growth of the cells was obviously inhibited ,the cells atrophied even turned circle, the amount of the cells decreased obviously. -7 2）When the concentration of hydrocortisone was between 10 and -3 10 g/L, it showed an increasing tendency of the content of melanin when the concentration of hydrocortisone increased，when it was 10-3g/L, the content of melanin significantly increased comparing with the control; while the concentration was between