姜黄素对人胃癌MGC823细胞凋亡影响.docVIP

姜黄素对人胃癌MGC823细胞凋亡影响[摘要] 目的：探讨姜黄素对人胃癌MGC823细胞体外增殖及凋亡的影响。方法：选用人胃癌MGC823细胞进行体外培养，采用MTT法测定不同浓度的姜黄素对MGC823细胞的增殖抑制作用，流式细胞仪监测凋亡；荧光显微镜技术和电镜技术，观察姜黄素对细胞诱发凋亡及细胞周期的变化。结果：姜黄素可明显抑制细胞的生长，其抑制率与药物浓度成剂量依赖关系。流式细胞分析仪结果表明，姜黄素能使MGC823细胞在加药后48 h出现凋亡峰。电镜观察可见姜黄素能使细胞发生凋亡，出现核边集及染色质浓缩成块状。结论：姜黄素可抑制Ec-9706细胞的生长，其机制可能与诱导细胞凋亡有关。 [关键词] 姜黄素；MGC823细胞；凋亡；胃癌 [中图分类号] R735.2 [文献标识码]A[文章编号]1674-4721（2011）06（b）-026-02 Effects of curcumin-induced apoptosis in human colon carinoma cells WANG Ping, CHEN Junlin Luohe Medical College, Henan Province, Luohe 462002, China [Abstract] Objective: To observe the effects of curcumin on proliferation and apoptosis in the human gastric carcinoma MGC823 cells． Methods: Methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation of MGC823 cells． The cell apoptos was and changes of cell cycle ofMGC823 cells were analyzed by using flow cytometry. Results: Curcumin significantly inhibited the ceil proliferation of MGC823 cells in dose, dependent manner． The results of flow cytometry showed that an apoptotic peak was found after MGC823 cells treated with curcumin for 24 h． Condensed and highly fluorescent granular cell nuclei under fluorescence microscopy and chromatin condenstation and aggregation on the nuclear margin under electron microscopy were observed in MGC823 cells treated with curcumln． Conclusion: Curcumln would inhibit the growth of colon cancer cells and the mechanism responsible for the effect might be involved in the induction of apoptosis. [Key words] Curcumin；MGC823 cells；Apoptosis; Gastric cancer 胃癌是常见恶性肿瘤，预后较差，是人类的主要肿瘤死因之一。姜黄（curcuma longa）是姜科植物姜黄的根茎，姜黄主要成分姜黄素具有广泛的药理作用，姜黄素对多种肿瘤细胞具有细胞毒作用,可以抑制肿瘤细胞生长和诱导其凋亡，具有抗氧化、抗肿瘤、抗感染等多方面作用，尤其是它的抗肿瘤研究备受关注。有研究表明姜黄素能对多种肿瘤发挥肿瘤抑制作用[1- 2]，但其具体作用机制尚不明确。本实验通过不同浓度的姜黄素作用于人胃癌MGC823细胞，探讨对其增殖及促凋亡的影响。 1 材料与方法 1.1材料 MGC823细胞由郑州大学病理生理学教研室提供，姜黄素系英国 DAH 公司产品，PMI1640、四氮甲唑蓝（MTT）、二甲亚砜（DMSO）、碘化丙啶（PI）系美国 Sigma 公司产品。姜黄素购自 Sigma公司，引物购自Takara公司。 1.2 方法 1.2.1人MGC823细胞培养人MGC823细胞置于完全培养基（10