bcr-abl基因疫苗对小鼠SP2-0-bcr-abl移植瘤影响.docVIP

bcr-abl基因疫苗对小鼠SP2/0/bcr-abl移植瘤影响 　 作者：姜扬文，钱莉，蒋桂花，刘伟，龚卫娟，季明春 毕业论文 【摘要】; 　　为了研究bcr-abl融合基因疫苗对小鼠SP2/0/bcr-abl移植瘤的影响，采用pVbcr-abl 、pVbcr-abl/mIL7两种质粒分别免疫BALB/c小鼠，然后用SP2/0/bcr-abl细胞攻击免疫小鼠，观察疫苗对SP2/0/bcr-abl移植瘤生长的影响，检验其是否具有免疫保护作用。结果表明：用基因疫苗免疫的两个实验组与两个对照组（空白对照组和空载体对照组）相比，在移植肿瘤形成时间、肿瘤表面破溃时间、肿瘤体积、荷瘤生存期等指标上均存在明显差异。pVbcr-abl/mIL7免疫小鼠组的荷瘤生存时间比pVbcr-abl免疫组相对延长。常规病理学分析发现，对照组小鼠的移植瘤组织比较致密，瘤细胞体积较大，形状不规则，而两个免疫组的肿瘤组织较为疏松，肿瘤细胞体积相对较小，并有大量炎性细胞浸润。两个对照组小鼠的肝脏组织内发现有大量肿瘤转移灶，而两个免疫组小鼠则未发现。结论： 接受 bcr-abl 基因疫苗免疫的小鼠被诱导产生较强的特异性免疫保护力，对 SP2/0/bcr-abl 移植瘤的体内生长有一定抑制能力。 毕业论文 【关键词】; 慢性粒细胞白血病；bcr-abl融合基因；基因疫苗；SP2/0/bcr-abl移植瘤 毕业论文 　　Influences of bcr-abl Gene Vaccine on; Inoculated SP2/0/bcr-abl Tumor Cells in Mice 毕业论文 　　Abstract ; To study the; influence of; vaccine of bcr-abl fusion gene fragment on inoculated SP2/0/bcr-abl tumor cells in mice，BALB/c mice were immunized with pVbcr-abl，pVbcr-abl/mIL7 plasmids，respectively，then SP2/0/bcr-abl cells; expressing the fragment of bcr-abl fusion gene were; inoculated subcutaneously into the groin of BALB/c mice in order to observe the effect of vaccine on growth of inoculated SP2/0/bcr-abl tumor cells.; The results showed; that there were distinct differences on the; time of tumor growth，the time of tumor ulceration，tumor; volume and survival time of mice bearing tumor; between two immunized groups and two control groups (blank and vacant plasmid groups). The mice immunized with pVbcr-abl/mIL7 lived longer as compared to mice immunized with; pVbcr-abl. The tissue of inoculated tumor was more compact，tumor organ; was larger，tumor form; was; irregular in 2 control groups，while the tissue of inoculated tumor was looser，tumor volume was smaller，and with mass inflammatory infiltration in two immunized groups. Moreover， the metastatic; tumor cells were found in the livers of control groups，but not observed in two immunized groups. It is concluded; that the protection; occured in immunized mice; which; inhibited the growth of SP2/0/bcr-abl tumor cell i