IL28B mRNA在小鼠纤维化肺组织内表达及意义.docVIP

IL28B mRNA在小鼠纤维化肺组织内表达及意义 　 作者：范利娟， 郑金旭， 徐丽娜 【摘要】 目的： 研究白介素28B(IL28B)mRNA在小鼠肺纤维化模型中的表达，探讨其在肺纤维化发病过程中的意义。方法： 健康昆明种雄性小鼠72只，随机分成3组：对照组(生理盐水)、模型组(博莱霉素)和治疗组(博莱霉素+泼尼松)，每组24只。第7，14，21，28天各组处死小鼠6只。无菌操作下取出小鼠肺组织，RTPCR检测IL28B的mRNA表达。结果： 第7，14，21天时，模型组IL28B的mRNA表达显著高于对照组(Plt;0.05，Plt;0.01，Plt;0.01);其中模型组14天时，IL28B表达最高。第7，14，21天时，治疗组IL28B的mRNA表达显著低于模型组(Plt;0.05，Plt;0.01，Plt;0.01)。结论： IL28B参与了肺纤维化的发生、发展过程，在肺纤维化的早期发挥了重要作用。 【关键词】 白介素28B; 干扰素λ3; 肺纤维化 　　[Abstract] Objective: To determine the expressions of Interleukin 28B(IL28B)in lung tissues of mice with pulmonary fibrosis(PF) induced by bleomycin at various periods and to study the effects of the cytokine in the pathogenesis of pulmonary fibrosis.Methods： Seventy two male mice were randomly divided into three groups: control group(n=24), model group(n=24) and therapy group(n=24).The mice of control group were treated with normal saline by intratracheal instillation, while the mice of model group and therapy group treated with bleomycin solutions.From the first day after bleomycin challenge, the therapy group was perfused into stomach prednisone daily.On day 7, 14, 21, 28 after administration, 6 mice of each group were sacrificed and the lung tissues were harvested.The expressions of IL28B mRNA in lung tissue of mice were detected by method of RTPCR.Results： The expressions of IL28B mRNA detected in lung tissue of model group were higher than that of control group on the 7，14，21th day, and peaked on the 14th day.The expressions of the therapy group on the 7，14，21th day were lower than that of the model group.Conclusion： IL28B may contribute to the pathogenesis of pulmonary fibrosis in early period. 　　[Key words] interleukin 28B; interferon lambda 3; pulmonary fibrosis 　　肺间质纤维化(pulmonary fibrosis，PF)是一种肺部异质性疾病，病理特点主要表现为弥漫性肺泡炎、肺泡单位结构紊乱和肺纤维化。特发性肺纤维化(idiopathic pulmonary fibrosis，IPF)是其中病因不明、进展快、具有致死性的一类疾病。目前, IPF的发病率在全球呈上升趋势，其发病机制尚不明确，而细胞因子的网络调控在其中占了重要的作用。IL28B是2003年发现的新型细胞因子，又称干扰素λ3(interferon lambda 3