IVIG 治疗对复发性流产患者外周血NK细胞影响.docVIP

IVIG 治疗对复发性流产患者外周血NK细胞影响 　 作者：李维茹 谭剑平 陈慧 刘玉昆 王振花 王蕴慧 张睿 刘颖琳 张建平 【摘要】 【目的】 探讨静脉滴注免疫球蛋白(IVIG)治疗对复发性流产患者(RSA)外周血NK细胞的影响。【方法】 研究组包括31例RSA患者，均进行静脉滴注免疫球蛋白(IVIG)治疗，于治疗前后采用流式细胞术检测患者外周血NK细胞毒性及NK细胞在所有淋巴细胞中的比例;26例未接受IVIG的RSA患者为对照组，首次检测检测NK细胞毒性及NK细胞比例后，于第10天复查上述项目。【结果】 治疗前研究组NK细胞毒性为(19.7 ± 8.7)%，NK细胞比例为(21.4 ± 8.2)%，治疗后实验组NK细胞毒性为(13.1 ± 3.7)%，NK细胞比例为(19.3 ± 7.5)%;对照组第一次检测，NK细胞毒性为(20.1 ± 7.0)%，NK细胞比例为(21.0 ± 8.1)%，间隔10 d复测，NK细胞毒性为(19.8 ± 7.2)%，NK细胞比例为(21.2 ± 7.7)%。研究组患者IVIG治疗前后外周血NK细胞毒性及比例显著降低，差异有统计学意义(P lt; 0.05)，而对照组前后两次外周血NK细胞毒性及比例无显著差异(P gt; 0.05);研究组患者IVIG前后外周血NK细胞毒性改变与IVIG前后NK细胞比例改变没有明显的相关性(P gt; 0.05)。【结论】 IVIG治疗对NK细胞毒性及比例产生降调节作用。 【关键词】 复发性流产; 静脉滴注免疫球蛋白; NK细胞毒性; NK细胞比例 　　Abstract： 【Objective】 To discuss the influence of intravenous immunoglobulin (IVIG) on peripheral blood natural killer cells in women with recurrent spontaneous abortion. 【Methods】 There are 31 patients with a history of RSA (recurrent spontaneous abortion) received the IVIG. Maternal peripheral blood samples were taken from these patients and the NK cell cytotoxicity and percentage were detected before and after the treatment by flow cytometric analysis. Twenty control subjects were not receive the treatment. 【Results】 Before the treatment， the NK cell cytotoxicity in experimental group was (19.7 ± 8.7)% and that was (20.1 ± 7.0)% in control group. After the treatment， the NK cell cytotoxicity in experimental group was (13.1 ± 3.7)% and that was (19.8 ± 7.2)% in the control group. Before the treatment， the NK cell percentage in experimental group was (21.4 ± 8.2)% and that was (21.0 ± 8.1)% in control group. After the treatment， the NK cell percentage in experimental group was (19.3 ± 7.5)% and that was (21.2 ± 7.7)%in the control group. In experimental group， the NK cell cytotoxicity and percentage were significantly reduced (P lt; 0.05) and that in control had not significant difference (P gt; 0.05). In experimental group， the change of NK cell cytotoxicity and the change of NK cell percentag