rhG-CSF动员对供者血浆S1P浓度影响.docVIP

rhG-CSF动员对供者血浆S1P浓度影响 　 作者：黄文荣，王立生，段海峰，高春记，鲁茁壮，王华，达万明 【摘要】 　　1-磷酸鞘氨醇（S1P）是具有广泛生物学效应的磷脂代谢产物，多种生长因子均能通过影响细胞鞘氨醇激酶（SphK）活性而调节S1P的生成，重组人粒细胞集落刺激因子（rhG-CSF）是一种广泛用于外周血造血干细胞动员的一种细胞因子。本研究主要了解rhG-CSF动员对供者血浆S1P含量的影响。分离供者rhG-CSF动员前和动员第5天外周血单个核细胞和血浆，用RT-PCR检测动员前后单个核细胞SphK mRNA表达情况，并应用γ-32P-ATP掺入法和SphK酶促反应检测动员前后血浆S1P变化。结果表明，供者动员前后外周血单个核细胞均表达SphK mRNA；在rhG-CSF动员前后供者血浆中均含有S1P，且S1P浓度在rhG-CSF动员前和动员后第5天无明显变化（Pgt;005）。结论： rhG-CSF动员对供者血浆S1P含量无明显影响。 【关键词】 重组人粒细胞集落刺激因子；外周血动员；血浆；1-鞘氨醇激酶 　　Impact of rhG-CSF on Sphingosine 1-phosphase Concentration in Blood Plasma of Donors 　Abstract Sphingosine 1-phosphate (S1P)，which can be impacted by different growth factors through sphingosine kinase (SphK)，is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Recombinant human granulocyte-colony-stimulating factor（rhG-CSF） have been used widely in peripheral blood stem/progenitor cell mobilization. This study was aimed to investigate the effects of rhG-CSF on S1P concentration in plasma of donors. The peripheral blood mononuclear cells and blood plasma were collected from 8 peripheral blood progenitor cell donors before mobilization and on the fifth day of mobilization with rhG-CSF. The SphK mRNA expression of blood mononuclear cells were detected by RT-PCR. The changes of S1P concentration were assayed with SphK enzyme catalyzed reaction. The results showed that both kinds blood mononuclear cells collected before and after rhG-CSF mobilization expressed SphK mRNA. The S1P concentration is low in donor’s plasma both before and after mobilization with rhG-CSF，and there was no markedly change of S1P concentration in plasma before and after mobilization（Pgt;0.05）. In conclusion，mobilization with rhG-CSF does not impact S1P concentration in donors plasma. 　　Key words rhG-CSF peripheral blood; Mobilization; plasma; sphingosine 1-phosphate 　　体内1-磷酸鞘氨醇（sphingosine 1-phosphase，S1P）主要来源于细胞膜鞘磷脂的分解代谢，参与S1P生物合成调节的关键限速酶为鞘氨醇激酶（sphingosine kinase，SphK）［1］。人外周血小板、红细胞、中性粒细胞和单个