rhG-CSF对健康供者外周血及骨髓免疫特性影响比较.docVIP

rhG-CSF对健康供者外周血及骨髓免疫特性影响比较 　 作者：赵翔宇，常英军，黄晓军 【摘要】 　　本研究探讨rhG-CSF对健康供者外周血采集物（G-PB）和骨髓采集物（G-BM）免疫学特性影响的异同。用MTT法和夹心酶联免疫复合物（ELISA）法检测G-PB和G-BM中T淋巴细胞增殖能力和IL-4、IFN-γ的分泌，并用流式细胞术测定两种移植物的T细胞亚群、树突状细胞（DC）亚群、单核细胞及共刺激分子CD28的表达。结果表明： G-PB中淋巴细胞，CD3+、CD4+、CD8+ T淋巴细胞，DC1、DC2以及单核细胞的含量，CD4/CD8的比值高于G-BM（P＜0.001）。G-PB的T淋巴细胞增殖能力高于G-BM（P＜0.05）；每微升G-PB移植物中T淋巴细胞分泌细胞因子IFN-γ、IL-4的量均明显高于G-BM，且G-PB中IL-4/IFN-γ比值小于G-BM（P＜0001），DC2与T淋巴细胞的比值也低于G-BM（P＜0.01）；而G-PB中CD4+、CD8+细胞上CD28表达的百分比和总体表达均高于G-BM（P＜0001）。结论： rhG-CSF体内应用诱导G-PB和G-BM产生的T细胞免疫低反应性是有差异的，而两者之间的差异是G-PB和G-BM移植后移植物抗宿主病（GVHD）发生率和程度不同的免疫学基础。 【关键词】 粒细胞集落刺激因子；外周血移植物；骨髓移植物；共刺激分子；免疫低反应性 　　Effects of rhG-CSF Mobilization on Immunological Properties of Grafts from Peripheral Blood and Bone Marrow 　　Abstract This study was aimed to investigate the difference of immunological properties between recombination human granulocyte colony-stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB) and rhG-CSF primed bone marrow grafts (G-BM).The lymphocyte proliferation ability and the quantities of interleukin-4 (IL-4) and interfe-ron-γ (IFN-γ) secreted by T cells were determined by using MTT assays and sandwich ELISA; T cell subgroups，dendritic cells (DC)，monocytes and the expression of CD28 costimulatory molecules on T cells were determined by multicolor flow cytometry. The results showed that the absolute numbers of lymphocytes，monocytes，CD3+，CD4+ and CD8+ T cells as well as DC1 and DC2，the ratios of CD4/CD8 in G-PB were significantly higher than those in G-BM，respectively (P＜0.001). T cell proliferation ability was significantly higher in G-PB than that in G-BM (P＜0.05). The quantities of IFN-γ and IL-4 secreted by T cells per micromilter of G-PB was significantly higher than those of G-BM，the ratios of IL-4/IFN-γ were significantly lower in G-PB than that in G-BM (P＜0.001). As compared with G-BM，the ratio between DC2 and T-lymphocyte was significantly low in G-PB (P＜0.01)，whereas the percentage and overall expression of CD28 on CD4+ and C