S100A4蛋白在47例人肺腺癌中表达及意义.docVIP

S100A4蛋白在47例人肺腺癌中表达及意义 　【摘要】 　　［目的］研究钙离子结合蛋白A4（S100A4）在肺腺癌组织、癌旁肺组织和正常肺组织中的表达及其临床意义。［方法］应用免疫组化SP法检测47例肺腺癌及相应癌旁肺组织标本（距肿瘤3cm~5cm）、15例正常肺组织标本中S100A4蛋白表达。［结果］在正常肺组织中S100A4蛋白无表达；在癌旁肺组织中S100A4蛋白表达率为12.8%（6/47）；在肺腺癌组织中的表达率为59.6%（28/47），与正常及癌旁肺组织比较有显著性差异（Plt;0.05）；S100A4蛋白在肺腺癌中的表达与淋巴结转移及癌细胞分化程度均有相关性（Plt;0.05）。［结论］S100A4蛋白的表达与肺腺癌的发生和淋巴结转移正相关，与肺腺癌分化程度负相关。 【关键词】 肺腺癌　S100A4蛋白　免疫组织化学 　　Expression of S100A4 Protein in 47 Cases with Human Lung Adenocarcinoma and its Clinical Significance 2. Liaoning Provincial Cancer Hospital, Shengyang 110042, China)Abstract: ［Purpose］ To investigate the expressions of S100A4 protein in lung adenocarcinoma tissues, cancer around tissues and normal lung tissues and their clinical significance.［Methods］The expression of S100A4 protein was detected by SP immunohistochemistry in 47 cases with lung adenocarcinoma and cancer around tissues (approximately 3~5cm from the cancer tissues), 15 cases with normal lung tissues. ［Results］There was no S100A4 expression in normal lung tissues.The positive expression rates of S100A4 were 12.8%（6/47） in cancer around tissues and 59.6%（28/47）in lung adenocarcinoma tissues. There was significantly difference between lung adenocarcinoma and normal tissue or cancer around tissues （Plt;0.05）. The expression of S100A4 protein in lung adenocarcinoma was associated with both differentiation grade and lymph node metastasis. ［Conclusions］Expression of S100A4 protein was positively related with the occurrence or lymph node metastasis of lung adenocarcinoma, and negtively related with its differentiation. Key words: lung adenocarcinoma; S100A4 protein; immunohistochemistry 多种恶性肿瘤的发生、侵袭和转移等病理过程密切相关[2]。本研究采用免疫组织化学方法检测15例正常肺组织，47例肺腺癌及癌旁肺组织标本S100A4蛋白的表达情况，并结合临床进行比较分析。 1 材料与方法 1.1 研究对象 本实验标本选自解放军第二0二医院及辽宁省肿瘤医院胸外科2005年3~9月手术切除的47例肺腺癌及癌旁肺组织标本（距肿瘤3cm~5cm），15例正常肺组织标本（均来自手术切除肺良性病变的周边肺组织），所有病例均未经过术前放、化疗。其中男性13例，女性34例；高-中分化30例，低分化17例；有纵隔淋巴结转移（N2）29例，无纵隔淋巴结转移（N0~1）18例。所有标本均经病理学确认，淋巴结分级采用1997年UICC标准。 1.2 试 剂 S100A4免疫组化用兔抗人多克隆抗体，购自NE