PAI-1 4G-5G基因多态性及严重急性呼吸综合征后骨坏死.docVIP

PAI-1 4G/5G基因多态性及严重急性呼吸综合征后骨坏死 　【摘要】 测定严重急性呼吸窘迫综合征（SARS）后骨坏死患者低纤溶倾向的纤溶酶原激活抑制物PAI和相关基因PAI-4G/5G基因多态性与国人SARS后骨坏死的关系，以便于非创伤性骨坏死的早期诊断和易感人群的筛选。［方法］取61例SARS后骨坏死患者抽取空腹肘静脉血。另取健康人群52名为对照，应用凝血仪、酶联免疫吸附（ELISA）方法测定PAI值，应用PCR+固相寡核苷酸连接分析（SPOLA）法测定PAI-1基因4G/5G多态性。［结果］SARS后骨坏死患者PAI异常明显PAI（1564±1385） U/ml VS（796±427） U/ml ，PAI-1基因4G/4G在骨坏死人群中增高，但统计学未见差异性，血浆PAI活性与4G/4G纯合子基因型密切相关。［结论］SARS后骨坏死患者的凝血指标异常，PAI可作为骨坏死易感因素的筛选指标。PAI-1基因4G/4G可能是国人骨坏死的遗传标记。 【关键词】 骨坏死 纤溶酶原激活抑制物 Abstract ［Objective］To explore the correlation between and post-severe acute respiratory symptom(SARS) patients with osteonecrosis,investigate the etiology of post-SARS osteonecrosis,and select the sensitive molecular symbols for early diagnosis and distinguish the high risk population［Method］ The studied subjects were divided into two groupsTotally 62 post-SARS patients with osteonecrosis was one group,and 52 age and sex matched healthy people was as normal controlled groupEmpty stomach blood samples from cubital veins were collected from both groupsPlasminogen activator inhibitor(PAI) by means of evzyme linked immunosorbent assay(ELISA),and PAI-1 4G/5G polymorphism was detected by polymerase chain reaction PCR and Solid phase oligonucleotide assay(SPOLA)［Result］ The blood agents of post-SARS patients changed obviously with PAI-1 polymorphism detected in post-SARS group was more than that of the control group,but had no statistical significanceThe plasma PAI activity was related to homozygote 4G/4G genotypeThis reveals that homozygote 4G/4G genotype maybe a susceptible gene mark to Chinese osteonecrosis patients［Conclusion］ PAI-1 is sensitive blood symbol for screening high-risk susceptible population4G/4G PAI-1 genotype may be an etiological factor in osteonecrosis Key words：osteonecrosis;SARS;plasminogen activator inhibitor(PAI) 非创伤骨坏死的发病机制中，各种潜在危险因子激活的血管内凝血使骨内血栓形成和骨坏死，有大量文献报道骨坏死的发生与患者的高凝低纤溶状态有关［1，2］。而纤溶酶原激活抑制物PAI及其因基4G/5G多态性在动静脉血检形成中起重要作用。作者对SARS后骨坏死患者的PAI及其基因4G/5G多态性进行检测，探讨其与骨坏死发生是否具有相关性，为骨坏死的早期诊断和干预提