p53对缺血再灌注损伤后肾小管上皮细胞演变影响.docVIP

p53对缺血再灌注损伤后肾小管上皮细胞演变影响 　 作者：李开龙, 杜晓兰, 宋瑞华, 赵玲, 何娅妮, 张建国 ;陈林 毕业论文 【关键词】; 缺血再灌注;,肾小管上皮细胞;,衰老;,凋亡;,p53 毕业论文 　　　[Abstract]; AIM: To observe the variation of renal tubular epithelial cells in p53（+/+） and p53（/）mice with young or old age at different time after kidney ischemia/reperfusion injury (IRI), and to investigate the; contribution of p53 gene in the variation. METHODS: p53（+/+） and p53(/) male mice at age of 2 and 12 months were made ischemic by clamping left renal hila for 45 min. At 0, 1, 3 and 7 d, 1, 3 and 6 month after reflow, renal tissues were processed for morphometric observation and proliferating cell nuclear antigen（PCNA）, apoptosis and senescenceassociated βgalactosidase (SAβgal) analysis, using hematoxylin and eosin stain, immunohistochemistry, terminal deoxynucleotidyl transferasemediated dUTPbiotin in situ nickend labeling (TUNEL) and histochemical staining, respectively. RESULTS: Renal tubule necrosis was more severely in p53(/) mice and aged mice compared to p53（+/+） mice and young mice (Plt;0.05), respectively. Apoptotic cells in p53（+/+） mice increased obviously compared to p53(/) mice (Plt;0.05) at 7 d after IRI.; In young wildtype mice, occasionally faint staining for SAβgal activity began to appear at 1 month, and obviously significantly increased at 3 and 6 months after IRI (Plt;0.05), but in contralateral kidney at any time as mentioned above, and in the IRI kidneys in p53(/) mice at 1 and 3 months, there; was almost no positive staining for SAβgal; occasionally positive staining for SAβgal was observed in the IRI kidney in p53(/) mice at 6 months after IRI. In p53 (/) and p53（+/+） aged mice, both kindeys had positive staining for SAβgal activity at 0d after IRI, but the level of the activity in p53(/) mice was much more lower than that in p53(+/+) mice (Plt;0.05), then the level of the activity; decreased notably at 1d in the IRI kidney (Plt;0.05). Positive stain of nuclear PCNA in p53（+/+） young mice had no statistical significan