PCNA及MMP9在宫颈癌组织中表达及意义.docVIP

PCNA及MMP9在宫颈癌组织中表达及意义 　 作者：孙红，王浩，秦天洁，阮之平，马瑾璐 【关键词】 宫颈肿瘤;增殖细胞核抗原；明胶酶B；免疫组织化学 　　Expressions and significance of PCNA and MMP9 in cervix cancer tissue 　　【Abstract】 AIM: To investigate the expressions of PCNA and MMP9 in cervix cancer tissue and their significance. METHODS: The immunohistochemical SP staining method was used to evaluate the PCNA and MMP9 expressions in 46 cases of cervix cancer tissues and 10 cases of normal cervix tissues. RESULTS: The expression of PCNA in cervix cancer tissues was much higher than that in normal cervix tissues (P=0.000)；The positive rate of MMP9 protein 82.61% in 46 cases of cervix cancer was significantly higher than that in 10 cases of normal cervix (30%, Plt;0.05). There was a positive correlation between PCNA and MMP9 expressions (rs=0.433, P=0.003). The expressions of PCNA and MMP9 were correlated with the clinical stage (Plt;0.05), but not with age, pathological stage or growth pattern (Pgt;0.05). CONCLUSION: The overexpression of MMP9 has a close relationship with the cell proliferation index in cervix cancer, suggesting that MMP9 may play an important role in the development of cervix cancer. 　　【Keywords】 cervix neoplasms; proliferating cell nuclear antigen (PCNA); gelatinase B; immunohistochemistry 　　【摘要】 目的： 探讨PCNA，MMP9在宫颈癌组织中的表达及临床意义. 方法： 用免疫组织化学SP法检测46例宫颈癌及10例正常宫颈中PCNA和MMP9的表达，分析不同组织中PCNA和MMP9表达的差异及两者的相关性. 结果： 宫颈癌组织PCNA的表达明显高于正常宫颈组织(P=0.000)；38例宫颈癌的MMP9阳性表达（82.61%）明显高于10例正常宫颈组织的表达(30%， P0.05)；PCNA的表达与MMP9的表达呈正相关（rs=0.433, P=0.003），且PCNA和MMP9均与临床分期有关（P0.05），而与年龄、病例分级及生长方式无关（P0.05）. 结论： MMP9在宫颈癌组织中表达异常增高，与细胞增殖关系密切，提示其对宫颈癌的发展起重要作用. 　　【关键词】 宫颈肿瘤;增殖细胞核抗原；明胶酶B；免疫组织化学 　　0引言 　　宫颈癌是最常见的女性生殖系统肿瘤，其局部侵袭力强，易直接蔓延和经过淋巴道转移. 从原位癌增殖到侵袭转移癌的演进过程,一方面依赖于肿瘤细胞本身的恶性潜能，另一方面是其实质与间质之间相互作用的结果. 增殖细胞核抗原（proliferating cell nuclear antigen, PCNA）是DNA聚合酶的辅助因子，能反映细胞增殖活性 . 基质金属蛋白酶9(matrix metalloproteinases9, MMP9)属于基质金属蛋白酶（matrix metalloproteinases, MMPs）家族成员，能降解Ⅰ, Ⅳ,Ⅴ型胶原及纤维粘连蛋白等基底膜和细胞外基质成分，从而促进恶性肿瘤的浸润、转移及间质血管新生；而间质血管新生则为肿瘤组织提供营养并排出其废物，使肿瘤得以持续迅速生长和发生浸润转移