β淀粉肽1-40在阿尔茨海默病病人脑内表达变化.docVIP

β淀粉肽1-40在阿尔茨海默病病人脑内表达变化 　【摘要】 目的 采用穹隆-海马伞切断及去势的大鼠模型模拟阿尔茨海默病病人，观察模型大鼠脑内不同部位β淀粉肽1-40(Aβ1-40)表达的变化，了解A β1-40与阿尔茨海默病（AD）相关的发病机制。方法 将大鼠分为穹隆-海马伞切断组、去势组（模型组）和假手术组，假手术组除了不切断穹隆-海马伞和去除卵巢外，其余步骤同模型组。常规处死大鼠取脑组织行A β1-40免疫细胞化学染色，观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynet核等部位Aβ1-40阳性细胞表达变化。结果 假手术组大脑各观察区有少量的Aβ1-40表达，与假手术组比较，模型组大脑海马CA1区、皮质区、杏仁复合体区、基底前脑Meynet核Aβ1-40阳性细胞数明显增多（t=3.969～6.677,Plt;0.01）。结论 穹隆-海马伞切断和去势的大鼠脑内多部位Aβ1-40表达增多，此可能与老年性痴呆病理改变有关。 【关键词】 穹隆 海马伞 淀粉样β蛋白 阿尔茨海默病 大鼠 Wistar 【Abstract】 Objective To observe the expressive varieties of β-amyloid（A β1-40）peptide in different sections of the rat brain with fimbria/fornix transaction and to study the pathogenic mechanisms related to Alzheimer disease(AD).Methods Health female Wister rats were randomly divided into the model group and control group with five rats in each group.The fimbria/fornix transection and ovaries cutting were simultaneously performed for the animal model.The rats were executed and the brain taken conventionly.The Aβ1-40 immunocytes were stained by using an immunohistochemical technique.The expressions of the Aβ1-40 positive cells in the hippocampi CA1,cortex,amygdale,Meynert of the basal forebrain were observed.Results A little A β1-40 was expressed in the observed sections of the brain in the control group,while the A β1-40 positive cells in the model group were significantly increased than those in the control group in the hippocammpiCA1,cortex,and amygdale(t=3.969～6.667,Plt;0.01).Conclusion Fimbria/fornix transaction cutting can cause the increased of Aβ1-40 expression in many sections of the brain, which might be related to the cognitive disturbance due to Alzheimer disease. 【Key words】 fornix; hippocampus fimbria; β-amyliod protein; Alzheimer disease;rats;Wistar β淀粉样肽是淀粉样蛋白前体蛋白（APP）的代谢产物，是老年斑的主要成分，其中以β-淀粉样肽1-40（Aβ1-40）为主。雌激素与APP的代谢过程密切相关［1］。阿尔茨海默病（AD）是一种以认知障碍为主要特征的进行性神经系统疾病，在美国AD发病年龄一般在65岁以上，80岁以上的患病率为40%，男女均可发病，但女性病人是男性的2～3倍，研究表明，女性AD病人体内的雌激素缺乏与AD发病有关［2］。大鼠卵巢切除（VOX，去势）是一种较好的雌激素缺乏模型［3］，结合穹隆-海马伞切断可以造成大鼠体内雌激素缺乏和脑内