不同宫内缺氧时程对胎鼠神经干细胞增殖影响及当归保护作用.docVIP

不同宫内缺氧时程对胎鼠神经干细胞增殖影响及当归保护作用 　 作者：陈滟　曹兵　梅欣明　曹坤　余鸿 【摘要】 目的探讨不同宫内缺氧时程对胚胎大鼠神经干细胞增殖的影响及当归注射液的保护作用。 方法将孕15 d的SD大鼠随机分为9组：对照组、缺氧3 h组、缺氧6 h组、缺氧9 h组、缺氧12 h组、当归3 h组、当归6 h组、当归9 h组和当归12 h组，每组5只。所有当归组孕鼠经尾静脉注入当归注射液，然后用低张性缺氧模型致胎鼠宫内缺氧；所有缺氧组用生理盐水代替当归注射液，其余同当归组；对照组只注盐水，不缺氧。取胎鼠大脑组织，作Nestin免疫组织化学染色、图像分析。结果缺氧3 h组、缺氧6 h组和缺氧9 h组阳性细胞较对照组染色增强，数量增多，缺氧12 h组阳性细胞较对照组减少，各缺氧组间比较有统计学差异；除当归12 h组外，其余各当归组阳性细胞较相应缺氧组染色减弱，数量减少；当归12 h组与缺氧12 h组间比较无统计学差异（P＞0.05）。结论一定时程、一定浓度的缺氧可以刺激神经干细胞进入增殖状态，其增殖的强弱与缺氧时程有关，缺氧6 h时达到增殖高峰, 缺氧9 h开始降低；长时程缺氧(12 h)会导致神经干细胞减少；当归对一定时程缺氧的胚胎大鼠神经干细胞有一定保护作用，而在缺氧12 h时失去保护作用。 【关键词】 神经干细胞； 缺氧；胚胎；增殖；当归 　　Abstract:ObjectiveTo study the influence of intrauterine hypoxia in different time on proliferation of embryo neural stem cells（NSE） and the protective effect of Angelica injection.MethodsThe pregnant SD rats in 15 days were randomly divided into 9 groups: control group (CG), hypoxia 3 h group(HG-3 h), hypoxia 6 h group(HG-6 h), hypoxia 9 h group(HG-9 h),hypoxia 12 h group(HG-12 h),Angelica group 3 h(AG-3 h), Angelica group 6 h(AG-6 h),Angelica group 9 h(AG-9 h),Angelica group 12 h(AG-12 h).Every group had 5 pregnant rats. The pregnant rats in Angelica groups were injected Chinese angelica injection via tail vein, and then the hypoxia model was used to cause embryo hypoxia in intrauterine. In hypoxia groups, 0.9% normal saline injection replaced Chinese angelica injecta, was injected in pregnant rats, and others were the same as Angelica group. The pregnant rats in control group were also injected 0.9% normal saline, but were not suffered hypoxia. After drawing materials, the cerebrum of rat embryo was carried out the immunohistochemical staining of Nestin.ResultsIn HG-3h, HG-6h,HG-9h,the immunoreactive positive cells of Nestin were deeper and more than the positive cells in CG. But the positive cells in HG-12 h were lighter and less than those in CG. And there had statistical difference between every hypoxia group. The positive cells of Nestin in AG-3 h were lighter and less than those in