中药“附归参汤”修复混合菌所致小鼠输卵管炎性狭窄病理改变.docVIP

中药“附归参汤”修复混合菌所致小鼠输卵管炎性狭窄病理改变 　 【摘要】 目的 观察中药“附归参汤”灌胃给药后是否可改善混合菌导致的小鼠输卵管炎性狭窄的病理改变。为临床 应用 “附归参汤” 治疗 输卵管炎所致的不孕症提供实验依据。 方法 昆明小鼠随机分为对照组（n=10）、模型组（n=10）、模型组+生理盐水处理组（n=10）、模型+“附归参汤”处理组（n=20），输卵管炎性狭窄模型采用混合菌（溶血性链球菌、大肠埃希菌和金黄色葡萄球菌2∶1∶1）输卵管接种法制作，处理组分别胃饲生理盐水和“附归参汤”30天。应用组织学方法观察各组的病 理学 改变，评判各处理组病理学转归情况。结果 在使用混合菌接种后，小鼠输卵管管壁结构发生改变，主要包括黏膜层水肿、上皮细胞顶端纤毛变短或消失、固有层炎性细胞浸润、毛细血管充血、管腔狭窄甚至闭塞。“附归参汤”长期给药后明显使上述病理改变向正常组织转归。结论 中药“附归参汤”能够修复混合菌导致的小鼠输卵管炎性狭窄的病理改变，为临床应用该药治疗输卵管炎所致的不孕症提供了实验依据。 【关键词】 中药;输卵管炎性狭窄;不孕;小鼠 毕业论文 【Abstract】 Objective Long time intragastric administration of “Fuguishen Tang” (FGST), a traditional Chinese medicine used in clinical practice, was investigated whether it recovered the pathological change of oviductal salpingitis and sterllity caused by bacteria. The aim was to attest an efficiency of FGST to treat the sterility caused by salpingitis for clinical therapy.Methods Kunming mice were random divided into three groups: control group (n=10), model group (n=10), model+saline treated group (n=10) and model + FGST treated group (n=20). The model group was induced by mixed bacteria (beta streptococcus, Escherichia coli and Staphylococcus aureus, ration 2:1:1). The treatment of saline and FGST were intragastrically administrated once per day for period of 30 days. The histological methods were used to observe the pathological alterations. Results The injection of mixed bacteria caused a pathological alteration of oviductal wall, such as edema in mucous, shortened cilia of epithelium, inflammatory cell infiltrating, dilation and hyperraemia in blood capillary within lamina propria and obstruction of oviductal lumen. These pathological changes were recovered following the treatment of FGST intragastrically administrated.Conclusion Mixed bacteria injected into oviduct in mice may induce salpingitis. Intragastric administration of FGST improves the pathological alteration of oviduct caused by bacteria 毕业论文 【Key words】 Chinese medicine;salpingitic stenosis;sterility;mic