中药天年饮对衰老大鼠学习记忆及脑单胺类神经递质含量影响.docVIP

中药天年饮对衰老大鼠学习记忆及脑单胺类神经递质含量影响 　 作者：陈志宏， 高福禄， 庞晓静 【关键词】 中药 　　摘要：目的观察中药天年饮（Tiannianyin，TNY）对D半乳糖衰老大鼠 学习 记忆及脑单胺类神经递质去甲肾上腺素（NE）、多巴胺（DA）、5羟色胺（5HT）含量的 影响 。 方法 选用成年雄性SD大鼠40只，随机分为4组，每组10只：正常组、衰老模型组、TNY用药组、阴性对照组。D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型，采用Morris水迷宫检测各组大鼠的空间学习记忆能力及采用高效液相色谱电化学法检测各组大鼠下丘脑、海马NE，DA，5HT的含量。结果D半乳糖衰老大鼠空间学习记忆能力明显下降并且衰老大鼠下丘脑，海马NE，DA，5HT的含量明显降低（与正常大鼠相比Plt;0.01）；TNY可明显改善衰老大鼠的空间学习记忆能力及提高脑单胺类神经递质的含量（Plt;0.01或Plt;0.05）。结论TNY可有效调整中枢神经递质的合成及提高学习记忆能力，具有良好的延缓衰老的作用。 毕业论文 　　关键词：天年饮；衰老；学习记忆；单胺类神经递质 　　Influence of Chinese Decoction TNY on Learning and Memory and Monoamine Neurotransmitter in Aging Rats Brain 　　Abstract:ObjectiveTo observe the influence of Chinese decoction Tiannianyin (TNY) on learning and memory and monoamine neurotransmitter contents in Dgal aging rats brain. Methods40 adult male SD rats were used in this study and divided into 4 groups at random: normal group, aging model group, TNY treatment group, and negative control group, each group had 10 rats. The subacutely aging rats were made by injecting Dgal into the rats’ abdominal cavity continuosly. The learning and memory ability of rats in each group were detected with Morris water maze. The monoamine transmitter contents in hypothalamus and hippocampus were detected with HPLCECD.ResultsThe learning and memory ability of aging rats dropped significantly compared with normal rats(Plt;0.01). The NE, DA, 5HT contents in hippocampus and hypothalamus of aging rats decreased significantly compared with normal rats (Plt;0.01). In TNY treatment group, the learning and memory ability and NE, DA, 5HT contents increased significantly compared with aging rats (Plt;0.01，Plt;0.05).Conclusion TNY can regulate the disorderly metabolized monoamine transmitter effectively and improve the learning and memory ability. Consequently, TNY has fairly antiaging effects. 毕业论文 　　Key words:TNY;Aging;Learning and memory; Monoamine neurotransmitter 　　脑内单胺类神经递质是一类调节机体生理活动的重要物质，这些物质在组织中的含量是反映神经递质生物合成、释放、摄取、失活等过程的生化指标；在 研究 神经元功