丹红注射液对睡眠剥夺大鼠学习记忆影响.docVIP

丹红注射液对睡眠剥夺大鼠学习记忆影响 　 作者：司江华，杨金升，石向群，曲传勇，张明磊，王彦平，范磊 【摘要】 目的探讨丹红注射液(Danhong injection)对睡眠剥夺(sleep deprivation, SD)大鼠学习记忆及海马胶质纤维酸性蛋白(Glial Fibriliary Acidic Protein，GFAP)表达的影响。方法用小平台水环境法建立SD模型。选用Wistar大鼠，根据SD时间的不同(0～5 d)，随机分为6组，每组又分为实验组和对照组，实验组给予丹红注射液，相应时间点对各组大鼠行Morris水迷宫测试，免疫组化法检测海马组织中GFAP的含量。　　结果①Morris水迷宫实验： 2，3，5 d组潜伏期，实验组低于对照组(Plt;0.05)，4 d组潜伏期，实验组低于对照组(Plt;0.01);2 ，5 d组游泳路程，实验组低于对照组(Plt;0.05)，3，4 d组游泳路程，实验组低于对照组(Plt;0.01);SD各时间点组穿越平台次数，实验组和对照组无统计学差异(Pgt;0.05)。②海马GFAP含量：2 ，3 d组海马GFAP含量，实验组高于对照组(Plt;0.05)，4，5 d组海马GFAP含量，实验组高于对照组(Plt;0.01)。结论丹红注射液对SD后大鼠学习记忆的损害有保护作用，这种作用可能与海马中星形胶质细胞(astrocyte，AS)功能活化有关。 【关键词】 丹红注射液;睡眠剥夺;学习记忆;胶质纤维酸性蛋白;Morris水迷宫 　Abstract：ObjectiveTo investigate the effect of Danhong injection on cognitive function and the content of glial fibriliary acidic protein (GFAP) in hippocampus of Wistar rats during Sleep Deprivation(SD).MethodsSD was induced in Wistar rats by employing “flower pot” technique. The 72 rats were randomly divided into six groups according to the time of SD(0-5 d), and every group was also divided into two subgroups: experimental group and control group. The behaviors of rats were studied in Morris maze test at given time. Then immunohistochemistry was used to detect the level of GFAP in hippocampus.　　ResultsCompared with control groups, the escape latency and swimming distouce of experimental groups induced in 2,3,4,5 d(Plt;0.05) were decreased.There was no obvious difference in the times across plot between the experimental groups and control groups (Pgt;0.05). Compared with control groups, the content of GFAP of experimental groups was increased on 2,3,4,5 d (Plt;0.05).ConclusionDanhong injection can improve the rat impairment of cognitive function induced by SD via the activation of astrocyt(AS) in hippocampus. 　　Key words： Danhong injection; Sleep deprivation; Cognitive function; Glial fibriliary acidic protein; Morris water maze 　　睡眠剥夺(sleep deprivation, SD) 是一种特殊的生理心理状态，可引起机体认知功能、情绪、行为及免疫功能等一系列改变[1]。既往有研究表明