人参总皂甙协同造血生长因子体外诱导CD34+造血干-祖细胞扩增及分.docVIP

人参总皂甙协同造血生长因子体外诱导CD34+造血干/祖细胞扩增及分 　 作者：王建伟，王亚平，王莎莉，姜蓉 【摘要】 为探讨人参总皂甙（total saponins of panax ginseng, TSPG）协同造血生长因子体外诱导CD34+造血干/祖细胞（HSC/HPC）扩增与分化的作用，收集人脐血、骨髓细胞并采用StemsepTM干细胞分选系统分离纯化CD34+ HSC/HPC，用不同剂量TSPG加入不同组合的造血生长因子进行培养，检测细胞总数、CD34+细胞和CD33+细胞比例及集落形成细胞总数（CFC）、粒系祖细胞(CFU-GM)数量变化。结果显示： 10-70 μg/ml TSPG均可不同程度地提高脐血细胞总数、CFC数和CD34+细胞数， 50 μg/ml是最佳刺激浓度，可使细胞总数、CFC数和CD34+细胞数分别增至(2470.5±79.96)×103、(53.96±4.29)×100%和(21.86±3.09)×100%; 20 μg/ml是液体培养诱导骨髓CD34+细胞向粒系分化的最佳浓度，可使细胞总数、CFU-GM和CD33+细胞分别增至(133.2±9.03)×103、(26.78±1.91)×100%和(16.98±1.73)×100%；甲基纤维素半固体培养检测显示，TSPG（10-50 μg/ml）均能提高CD34+细胞形成CFU-GM的集落产率，以TSPG 20 μg/ml效果最为明显。结论：合适剂量的TSPG能够促进CD34+造血干/祖细胞体外扩增与定向诱导分化。 【关键词】 人参总皂甙；CD34+造血干/祖细胞；细胞扩增； 细胞分化 　　Synergistic Effects of Total Saponins of Panax Ginseng in Combination with Hematopoietic Growth Factor on Proliferation and Differentiation of CD34+ Cells Ex Vivo 　　　AbstractTo investigate the effects of total saponins of panax ginseng (TSPG) in combination with hematopoietic growth factors (HGF) on proliferation and differentiation of CD34+ cells ex vivo, the purified CD34+ cells from cord blood and bone marrow were expanded by various concentrations of TSPG with combination of cytokines in liquid culture systems and the expanded cell number, CD34+ cell number, CD33+ cell ratio, the numbers of total CFC and hematopoietic progenitor cell number were detected. The results showed that TSPG (10-70 μg/ml) could raise the expanded cell number, CD34+ cell number, and the numbers of total CFC, TSPG 50 μg/ml was identified as the most potent stimulating concentration, and increased total nucleated cells to (2470.5±79.96)×103 、 CFC to (53.96±4.286)×100% and CD34+ cells to (21.86±3.094)×100%; TSPG (10-50 μg/ml) could raise the colony formation rate of CFU-GM, TSPG(20μg/ml) induced the best effect on granulocytopoietic differentiation committed of CD34+ cells. It is concluded that the optimal concentration of TSPG can promote CD34+ cells to proliferate and differentiate by cooperating with h