兔骨髓基质干细胞体外培养复合β－磷酸三钙进行胸椎后侧融合实验.docVIP

兔骨髓基质干细胞体外培养复合β－磷酸三钙进行胸椎后侧融合实验 　 作者：许卫兵 贾连顺 卢建熙 卢旭华 滕红林 顾宇彤 杨建东 陈长青 【关键词】 脊柱融合 摘要：［目的］评价β－磷酸三钙复合骨髓基质干细胞为骨移植替代物进行脊柱融合的效果。［方法］60只兔子分四组进行胸椎后路融合：Ⅰ组（组织工程化骨即β－TCP复合BMSCs），Ⅱ组（β－TCP＋自体红骨髓），Ⅲ组（单纯β－TCP），Ⅳ组（自体髂骨）。术后12周处死其余动物进行放射学、手工力学和组织学检查。［结果］从影像学和手工力学比较，Ⅰ组的融合率高于Ⅱ、Ⅲ、Ⅳ组且有统计学差异。组织学上，Ⅰ组新骨形成量较多，陶瓷微孔内部充满新生骨;Ⅱ组骨小梁形成量较Ⅰ组少, 为新生骨与纤维组织混杂；Ⅲ组陶瓷骨颗粒微孔内多为结缔组织，新生骨的量少，只见少量新生骨贴附于陶瓷骨表面; Ⅳ组在椎板与移植骨的交界处有稀疏的新生骨小梁形成。［结论］β－TCP复合BMSCs是脊柱融合自体骨的良好替代物。 　　关键词：骨髓基质干细胞；β－磷酸三钙；脊柱融合；骨移植替代物 　　Bone marrow stromalderived osteoblastsporous βtricalcium phosphate composites as bone graft substitute for posterior thoracic spinal fusion 　　Abstract：［Objective］To evaluate the efficacy of autogenous bone marrow stromalderived osteoblastsporous(BMSCs) βtricalcium phosphate(βTCP) composites as an alternative to autogenous graft materials in spinal fusion.［Method］Posterior thoracic spine fusion was carried out in 60 White New Zealand rabbits using one of the following graft materials:βTCP granules plus cultured MSCs(group Ⅰ);β－TCP granules plus fresh autogenous bone marrow(group Ⅱ); β－TCP granules alone(group Ⅰ); and autogenous bone graft(group Ⅳ). All the rabbits were killed 12 weeks after surgery to evaluate with radiography, CT, manual palpation and histologic analysis.［Result］The rate of fusion was significantly higher in group Ⅰ compared with group Ⅱ, group Ⅲ and group Ⅳ; In group I histological analysis showed abundance newly formed bone in contact with the implanted granules;In group Ⅱ there were a reduced amount of newly formed bone and abundant fibrous tissue; In group Ⅲ there were relatively little new bone formation and abundant fibrous tissue; In group Ⅳ, there were thin trabeculae of newly formed bone closed to the decorticated lamina and some fibrous tissue.［Conclusion］The results indicate that bone marrow stromalderived osteoblastβ TCP composites may provide an well alternative to autogenous graft materials for spinal fusion. 　　Key words：Bone marrow stromal cell; βtricalcium phosphate; Spinal fusion; Bone