外界条件改变对红花离体培养影响.docVIP

外界条件改变对红花离体培养影响 　 作者：杨晶，李天航 熊立东，付宏歧 李校堃 【摘要】 目的研究外界培养条件的变化对红花离体培养过程中不同发育阶段培养物的影响。方法以红花种子诱导的无菌苗子叶为外植体，分别接种至含有不同碳氮源、不同PH值、不同温度、不同湿度和不同光照的培养基中，观察其生长情况。结果通过实验筛选出蔗糖浓度为3%，KNO3浓度为MS培养基中KNO3浓度的2倍，最佳pH为6.2，红花离体培养体系在24℃，30%的相对湿度，16 h光照，8 h黑暗交替培养的条件下，最利于红花的分化。结论3%的蔗糖作为碳源，最适合愈伤组织的生长;2倍于MS培养基中的KNO3含量有利于愈伤组织的生长和不定芽的分化;pH值为6.2时，最适宜红花不同阶段培养物的生长;24℃为红花离体培养的最适温度，生根时的温度为21℃较好;湿度为50%时，适于愈伤组织鲜重积累，30%的湿度适于不定芽分化和生根;黑暗培养利于鲜重积累，16 h光照，8 h黑暗交替培养有利于不定芽的分化，8 h光照，16 h黑暗交替培养有利于生根。 【关键词】 红花愈伤组织; 不定芽; 蔗糖 ; 氮源; pH值; 温度; 湿度; 光照 　　Abstract：ObjectiveTo study the effect of changed outside culture conditions on the safflower at the different development stages of culture in vitro. MethodsThe safflower cotyledon induced by safflower seeds as explants were inoculated into medium with different carbon , nitrogen sources, PH, temperatures, humidity and light and its growth situation was observed. Results3%sucrose concentration, 2 times MS medium KNO3 concentration the optimal pH of 6.2 were selected, safflower in vitro culture system at 24 ℃, 30% relative humidity, 16 h light, 8 h dark cycle under the conditions of culture, the most beneficial to the flower differentiation were selected .Conclusion3% sucrose as a carbon source, the most suitable for callus growth; 2 times KNO3 content in MS medium in favor of the callus growth and differentiation of adventitious buds; pH value of 6.2, the best safflower cultures at different stages of growth; 24 ℃ for in vitro culture of safflower optimum temperature, take root when the temperature of 21 ℃ for the better; humidity of 50%, suitable for callus accumulation of fresh weight, 30% humidity suitable for differentiation of adventitious buds and root; dark culture conducive to the accumulation of fresh weight, 16 h light, 8 h dark turn of the culture is conducive to the differentiation of adventitious buds, 8h light, 16h dark turn of the training will help take root. 　　Key words： Safflower calli; Adventive bud; Sucrose; Nitrogen source pH; Temperature; Humidity; Il