多发性骨髓瘤、急性白血病患者骨髓基质细胞生长、免疫表型及白.docVIP

多发性骨髓瘤、急性白血病患者骨髓基质细胞生长、免疫表型及白 　 作者：庄俊玲，汪玄，张洁萍，武永吉 【摘要】 　　为了研究多发性骨髓瘤（MM）及急性白血病（AL）骨髓基质细胞（BMSC）的生长速度、免疫表型及白介素6（IL-6）水平，应用骨髓贴壁细胞培养法培养BMSC观察细胞的生长速度；用流式细胞术测定免疫表型、细胞周期；用ELISA方法测定BMSC培养体系中IL-6的水平。结果表明，AL患者原代BMSC的生长时间（17.3±7.8天）和传代周期（10.3±3.5天）均显著短于MM患者（分别为26.5±6.3天和16.5±4.1天）和正常人（分别为25.8±6.3天和17.5±2.4天），AL患者BMSC细胞周期S+G2%（17.4±3.6%）显著高于MM患者（8.5±2.2%）和正常人（8.9±2.3%）。3组中CD29、CD44均为100%表达，而CD138、CD34、CD54、CD56均不表达，CD106在3组中均部分阳性。在3组BMSC上清中，IL-6水平在MM组（1 288.5±736.7 pg/ml）显著高于AL组患者（859.3±203.1 pg/ml）和正常人组（850.9±129.5 pg/ml）。结论：MM、AL患者和正常人BMSC的生长速度、细胞周期中S+G2%和IL-6水平存在显著差异，所检测的抗原表达无差异。 【关键词】 多发性骨髓瘤；急性白血病；骨髓基质细胞；免疫表型；白介素6 　　Growth，Immunophenotype and Interleukin-6 Level of Bone Marrow Stromal Cells in Patients with Multiple Myeloma and Acute Leukemia 　　Abstract The aim of this study was to investigate the growth，immunophenotype and interleukin-6 (IL-6) level of bone marrow stromal cells (BMSC) in patients with acute leukemia (AL) and multiple myeloma (MM). BMSC was cultured by wall-adhesion method and the growth of BMSC was observed. The immunophenotype and cell cycle of BMSC were detected by flow cytometry. The level of interleukin 6 (IL-6) in BMSC culture system was detected by ELISA. The results showed that the primary （17.3±7.8 days）and continuous （10.3±3.5 days）growth cycle of BMSC in patients with AL were significantly shorter than those in patients with MM (26.5±6.3 and 16.5±4.1 days respectively)， and shorter than those in normal controls (25.8±6.3 and 17.5±2.4 days) respectively. Similarly，S+G2%（17.4±3.6%）of BMSC in patients with AL was significantly higher than those in patients with MM（8.5±2.2%）and in normal controls（8.9±2.3%）. All of the three groups showed positive antigen expressions with CD29 and CD44 were 100%，while CD138，CD34，CD54，CD56 positive were not expressed and CD106 was partially expressed positive. The supernatant IL-6 level of BMSC system in MM patients （1288.5±736.7 pg/ml）was significantly higher than those in AL patients（859.3±203.1 pg/ml）and normal cont