姜黄素防治细菌脓毒症小鼠实验探究.docVIP

姜黄素防治细菌脓毒症小鼠实验探究 　【摘要】 目的 探讨姜黄素对大肠埃希菌引起的细菌脓毒症小鼠的保护作用。 方法 昆明种小鼠90只随机分为对照组、模型组和姜黄素组,每组30只。姜黄素组腹腔注射姜黄素固体分散剂,每天1次,连续3 d。第4天,模型组和姜黄素组尾静脉注射041184大肠埃希菌标准菌悬液建立细菌脓毒症模型。8 h后,每组处死小鼠20只检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肿瘤坏死因子α(TNFα)和一氧化氮(NO)含量,观察肝、肾、肺和肠等脏器病理改变,余下小鼠观察24 h生存率。 结果 姜黄素组血清AST、ALT、TNFα和NO含量明显低于模型组(Plt;0.05),病理检查见脏器损害亦明显减轻;24 h存活率90%,明显高于模型组(50%)。 结论 姜黄素可减少细菌脓毒症小鼠TNFα、NO和氧自由基的产生,减轻其脏器病理损害。 【关键词】 姜黄素 休克 脓毒 大肠杆菌 疾病模型 动物 ABSTRACT: Objective To investigate the effects of curcumin(Cur) on bacterial sepsis induced by Escherichia coli(E.coli) in mice. Methods Sixty mice were divided into 3 groups at random: a control group, an infected group and a curcumin group. Cur group: intraabdominal injection of curcumin solid dispersant(200 mg/kg) was given for three days. The infected group: 078 E.coli(5 1010cfu/L, 10 mL/kg) was administrated by intravenous injection to create the bacterial sepsis mice model. 8 hours after the injection, the level of AST, ALT, and TNFα and NO of each group was measured and mice were sacrified to observe the pathological change of liver, ridney, lung and intestines. Results Curcumin can remarkably reduce the level of the AST and ALT in the serum of the bacterial sepsis mice induced by E.coli(Plt;0.05) as well as that of TNFα(Plt;0.05) and NO(Plt;0.05). Meanwhile, it can significantly decrease the pathological change of liver. Conclusion Curcumin can play an important role to protect the mice from bacterial sepsis by ways of cytotoxic effects of NO, TNFα and oxygen free radicals. KEY WORDS: curcumin; shock,septic; Escherichia coli; disease models,animal 福建医科大学学报 2008年7月 第42卷第4期侯君艺等：姜黄素防治细菌脓毒症小鼠的实验研究脓毒症是由严重细菌感染引起的全身炎症反应综合征的晚期失代偿表现,是严重烧伤、创伤和感染的常见并发症，进一步发展可导致脓毒症性休克、多器官功能障碍综合症(multiple organ dysfuction syndrome, MODS)。1980年以来,对于细菌脓毒症和MODS发生的分子机制的研究,特别是对脂多糖(LPS)信号转导通路的深入研究,引发一系列阻断内毒素信号通路和对抗各种单一介质的治疗制剂的产生。但迄今尚没有一种制剂能够显著降低脓毒症性体克的临床死亡率[1]。姜黄素为姜黄的主要活性成分,具抗氧化、抗炎等药理作用,能减少各种炎症介质的合成和分泌[2]。笔者采用大肠埃希菌制备小鼠细菌脓毒症模型,探讨姜黄素预防给药对细菌脓毒症的保护作用。 　　1 材料与方法 　　1.1 材料 　　1.1