姜黄素抑制哮喘大鼠气道平滑肌细胞TGFβ1表达探究.docVIP

姜黄素抑制哮喘大鼠气道平滑肌细胞TGFβ1表达探究 　　[摘要] 目的 观察姜黄素对哮喘大鼠气道平滑肌细胞（ASMC）增殖及TGFbeta;1表达的影响。 方法 将24只雄性SD大鼠分为对照组、哮喘组和姜黄素组。以卵清白蛋白（OVA）致敏激发建立哮喘大鼠气道重塑模型，图像分析技术测定支气管壁厚度（Wat）和平滑肌厚度（Wam），免疫组织化学法（IHC）测定肺组织 TGFbeta;1 蛋白表达。酶联免疫吸附法（ELISA）测定细胞培养液中TGFbeta;1含量，实时定量 PCR（Real-time PCR）检测ASMC中TGFbeta;1mRNA表达，CCK-8分析ASMC增殖程度。 结果 姜黄素组与哮喘组相比，Wat及Wam厚度减少（Plt;0.01），仍高于对照组（Plt;0.01）；IHC显示：姜黄素组肺组织中 TGFbeta;1蛋白表达与哮喘组比较明显减少（Plt;0.01），仍高于对照组（Plt;0.01）。姜黄素组大鼠ASMC中TGFbeta;1和TGFbeta;1mRNA表达均降低，与对照组比较差异有统计学意义（Plt;0.01）；姜黄素组ASMC增殖明显受到抑制（Plt;0.01）。 结论 姜黄素能抑制哮喘大鼠ASMC增殖，其机制与降低TGFbeta;1的表达有关。　　[关键词] 转化生长因子beta;1；大鼠；哮喘；气道重塑；姜黄素　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701（2016）19-0034-04　　[Abstract] Objective To study the role of curcumin on proliferation mechanism of airway smooth musle cell （ASMC） and TGFbeta;1 expression in asthmatic rats. Methods A total of 24 Sprague-Dawley（SD） rats were sensitized and challenged by OVA to establish asthmatic model were divided into three groups with 8 rats in each group. The total bronchial wall thickness（Wat） and the airway smooth musle thickness（Wam） were measured by image analysis system. The TGFbeta;1 expressions were determined by immunohistochemistry. Vitro experiments were conducted to determine the direct effect of curcumin on TGFbeta;1 expression by ASMC. Real-time PCR was employed to detect the expression of TGFbeta;1 mRNA levels. TGFbeta;1 concentrations were determined by sandwich enzyme-linked immunosorbent assay（ELISA）. Cell Counting Kit-8（CCK-8） was used to detect ASMC proliferation. Results Wat and Wam of curcumin group were significantly thiner than asthma group （Plt;0.01）； but those were significantly thicker than control group （Plt;0.01）. The TGFbeta;1 expressions in the curcumin group was significantly lower than those of the asthmatic group（Plt;0.01）； but they were higher than the control group （Plt;0.01）. Compared to control group， curcumin decreased the protein expressions and mRNA expressions of TGFbeta;1 of ASMC （Plt;0.01）， inhibited ASMC proliferation （P