小儿退热贴祛邪作用实验探究.docVIP

小儿退热贴祛邪作用实验探究 　【关键词】 小儿 　　摘要：目的观察小儿退热贴的祛邪作用，为临床 治疗 小儿外感发热提供实验依据。 方法 采用给药清除流感A型病毒感染小鼠体内病毒的方法，观察小儿退热贴对流感A型病毒感染小鼠的存活率、平均存活时间、肺指数及血凝滴度的 影响 ；体外抗流感病毒试验采用MTT法检测病毒抑制率；体外抑菌实验(MIC测定法)观察小儿退热贴对肺克杆菌、绿脓杆菌、金黄色葡萄球菌、乙型链球菌、肺炎球菌的抑制作用；采用给药治疗葡萄球菌感染小鼠的方法，观察小儿退热贴对葡萄球菌感染小鼠的死亡率的影响。结果小儿退热贴1.04 g#12539;kg1#12539;d1可提高流感病毒A型感染小鼠的存活率、延长其存活时间，肺指数和血凝滴度与病毒对照组相比较具有显著性差异；小儿退热贴在浓度5～1 280 μg/ml的范围内，随浓度的增加，对流感病毒的抑制率也增加，并表现出量效关系；小儿退热贴含生药量0.83 g/ml对肺克杆菌有较强的抑制作用、含生药量0.11 g/ml对绿脓杆菌有较强的抑制作用、含生药量0.06 g/ml对金黄色葡萄球菌有较强的抑制作用、对乙型链球菌和肺炎球菌没有抑制作用；小儿退热贴可降低金黄色葡萄球菌感染小鼠的死亡率，与模型对照组比较有显著性差异(P＜0.05)。结论小儿退热贴具有体内外抗病毒、抑菌的作用。 论文代写 　　关键词：小儿退热贴；抗病毒；抑菌 毕业论文 　　Experimental Study on Eliminating Pathogenic Effects of Infantile Umbilical Paste 　　Abstract:ObjectiveTo provide an experimental evident for clinical treating infantile fever, we observed the eliminating pathogenic effects of infantile umbilical paste.MethodsWe applied the method of giving the medicine to mice infected by influenza virus A to detect some indexes, including survival rate, mean survival time, lung index, hemagglutination titer, to evaluate the effects of eliminating virus in vivo; We applied MTT method to detect protect rate of virus in vitro; In another experiment, we evaluated the medicine inhibition to Klebsiella Pneumoniae bactedostatic，P.aeruginosa, acpococcus，beta-streptococcus，preumococcus in vitro. We gave infantile umbilical paste to mice infected by acpococcus to evaluate the medicine effects on mice mortality.Results The group of infantile umbilical paste (1.04 g/kg) can increase survival rate of mice infected by influenza virus prolong survial time, and its lung index and hemagglutinati on tier have a signincant difference compared with the group only treated with virus；Within the range of 5～1280 μg/ml， when the medicine concentration increased，protect rate on group of infantile umbilical paste (0.83 g/ml) has stronger inhibiting effects on Klebsiella Pheumoniae，0.11 g/ml has stronger inhibiting effects on P.aemginoSa，0.06 g/ml has strong