当归补血汤对荷瘤小鼠影响及对环磷酰胺化疗增效减毒作用.doc

当归补血汤对荷瘤小鼠影响及对环磷酰胺化疗增效减毒作用 　 作者：袁国红, 庞晓静 马鹤超 【摘要】 研究当归补血汤对肿瘤的抑制作用及其与免疫功能的关系，并探讨其对环磷酰胺（cytoxan, CTX）化疗的增效减毒作用。 方法：以荷EL-4瘤株C57BL/6纯系小鼠作为病理模型，皮下肿瘤接种（1×106个肿瘤细胞），7 d时随机分为4组，肿瘤模型组，当归补血汤（Danggui Buxue Tang, DBT）组［24 g/（kg·d）］，CTX组［7.5 mg/（kg·d）］，DBT+CTX组，另设正常对照组。每天游标卡尺测定肿瘤长短径，并观察荷瘤小鼠生存时间，以检测DBT的抑瘤作用；免疫学方法（迟发型超敏反应、空斑形成细胞、细胞毒T细胞及自然杀伤细胞的杀伤活性，TNF-α活性及外周血、骨髓有核细胞计数等）检测DBT的抑瘤作用与机体免疫功能的关系及其对CTX化疗的增效减毒作用。 结果：接受相应处理15 d后，与肿瘤模型组比较，DBT、CTX和DBT+CTX均可显著减缓小鼠肿瘤生长速度（Plt;0.05），明显延长荷瘤小鼠生存时间（Plt;0.05）。与肿瘤模型组比较，DBT组和DBT+CTX组各项免疫学指标增强（Plt;0.05），CTX组各项免疫学指标明显降低（Plt;0.05）。与CTX组比较，DBT+CTX组各项免疫学指标均有大幅度改善（Plt;0.05）。结论：DBT可减缓肿瘤生长速度，延长荷瘤小鼠生存时间，其抑瘤作用可能与其激活机体免疫功能作用有关，并对CTX化疗有增效减毒作用。 【关键词】 抗肿瘤药; 环磷酰胺; 免疫学; 小鼠 Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a compound traditional Chinese herbal medicine for supplementing blood, on tumor growth in tumor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as well as its synergic effect in reducing toxicity of cytoxan (CTX). Methods: Experiment was carried out in tumor-bearing mice after inoculation of EL-4 cells. The mice were randomly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group ［24 g/(kg·d)］, CTX-treated group ［7.5 mg/(kg·d)］ and DBT plus CTX-treated group, with another ten normal mice as control. Inhibitory rate of tumor growth, survival time, immune function and variability of blood cells were measured in the mice during the experiment. Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group grew slower than the untreated group (Plt;0.05). Murine survival time in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group was lengthened as compared with that in the untreated group (Plt;0.05). Compared with the untreated group, all kinds of immune indexes in the DBT-treated group and DBT plus CTX-treated group were significantly improved (Plt;0.05), while th