心肌梗死大鼠心脏ACE2基因表达意义.doc

心肌梗死大鼠心脏ACE2基因表达意义 　 作者：陈高，黄从新，赵庆彦，姚艳 【关键词】 大鼠；ACE2,mRNA；心肌梗死 　　Expression of ACE2 mRNA in rat myocardium after acute myocardial infarction and its significance 　　【Abstract】 AIM: To investigate the changes of the mRNA expression of ACE2 after acute myocardial infarction (AMI) in SD female rat. METHODS: Thirtysix SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RTPCR and analyzed semiquantitatively by imageanalysis system. RESULTS: Compared with sham operated group, the mRNA expression of ACE2 had no significant difference in the infract and noninfarct areas of operated group at day 1 after AMI (Pgt;0.05). The mRNA expression of ACE2 increased in the infract area compared with the noninfarct area at day 3 after AMI (Plt;0.01). At day 28, increase of ACE2 mRNA expression were also seen in the noninfarct area of AMI rats compared with the myocardium of control rats (Plt;0.01). CONCLUSION: The gene expression of ACE2 at mRNA level was significantly increased in the myocardium after AMI. 　　【Keywords】 rats; ACE2 mRNA; myocardial infarction 　　【摘要】 目的：研究大鼠ACE2的mRNA在急性心肌梗死（AMI）后大鼠心脏组织中的表达. 方法：将36只SD雌性大鼠按时间段随机分为3组（每组12只），每组再随机分为假手术组、手术组2个亚组（每组6只），应用RTPCR方法检测大鼠AMI后1，3，28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达，并以图像分析系统对其进行半定量分析. 结果：在AMI后1 d，与假手术组相比，手术组梗死区及非梗死区ACE2 mRNA水平均无差异（Pgt;0.05）. 在AMI后3 d，与手术组非梗死区相比，手术组梗死区ACE2 mRNA水平明显升高(Plt;0.01). 在AMI后28 d，与假手术组相比，手术组非梗死区ACE2 mRNA水平也明显升高(Plt;0.01). 结论：AMI后ACE2 mRNA表达增多. 　　【关键词】 大鼠；ACE2 mRNA；心肌梗死 　　0引言 　　血管紧张素转化酶2（ACE2）可能通过肾素血管紧张素醛固酮系统（RAAS）或通过对一些新型调节肽系统的调制作用来调节心血管的功能, 但是否参与AMI后机体对心肌缺血缺氧的调节反应还不清楚. 我们从基因转录水平（mRNA）观察SD大鼠AMI后梗死区及非梗死区的左心室肌细胞ACE2 mRNA表达量的变化. 　　1材料和方法 　　1.1材料总RNA抽提试剂盒(Trizol试剂盒)由Invitrogen公司提供, RTPCR试剂盒购自宝生物工程(大连)有限公司. ACE2引物及内参基因引物, 以及