慢性肾衰中医证型及血中内皮素、一氧化氮关系探析.doc

慢性肾衰中医证型及血中内皮素、一氧化氮关系探析 　【摘要】 目的 为了解血浆内皮素(ET)与一氧化氮(NO)在慢性肾衰患者及中医证型中的变化。方法 用放射免疫法测量血浆ET含量，用镉活化比色法测定血清NO含量。结果 150例慢性肾衰患者血ET显著高于正常人组(P＜0.01)，血ET中除脾肾阳虚与阴阳两虚组无显著差异外(P＞0.05)，其余各组均有显著差异（P＜0.01）。血ET含量在中医各证型中依次为：正常人组lt;脾肾气虚组lt;气阴两虚组lt;肝肾阴虚组lt;脾肾阳虚组lt;阴阳两虚组。血NO含量在中医各证型中依次为：脾肾阳虚组gt;阴阳两虚组gt;肝肾阴虚组gt;气阴两虚组gt;脾肾气虚组gt;正常组。在病例组中ET和NO呈显著正相关(P＜0.01)。结论 慢性肾衰患者血浆ET含量的增加可以作为判断肾衰严重程度的一个客观指标，血清NO含量的变化可以反映出脏腑病损的严重程度。 【关键词】 肾衰/诊断 辨证分型 内皮缩血管肽类/分析 一氧化氮/分析 Study on relationship between blood NO，ET amp; TCM syndromes 【Abstract】 Objective To investigate the changes in circulating endothelin(ET)and nitricoxide(NO)of chronic renal failure and TCM syndromes of chronic renal failure.Methods The level of plasma ET was measured using radio immuno assay， while the level of serum NO was measured using cadmium reduction bar chromatographic analysis and colorimetry.Results Compared with the control group， the plasma ET level was much higher in 150 patients with chronic renal failure (P＜0.01)， while the serum ET level did not show any significant difference between the deficiency syndrome of spleen and kidney-Yang groups and the deficiency syndrome of both Yin and Yang (P＞0.05).The rest of groups displayed the significant difference(Plt;0.01). The serum NO level was positively correlated with the plasma ET level (P＜0.01).The plasma ET level of different TCM syndromes decreased according to the following sequence： the control group， the deficiency syndrome of spleen and kidney-Qi， the deficiency syndrome of both -Qi and Yin， the deficiency syndrome of liver and kidney-Yin， the deficiency syndrome of spleen and kidney-Yang， the deficiency syndrome of both Yin and Yang； while the serum NO level increased according to the following sequence： the deficiency syndrome of spleen and kidney-Yang， the deficiency syndrome of both Yin and Yang ， the deficiency syndrome of liver and kidney-Yin， the deficiency syndrome of both Qi and Yin， the deficiency syndrome of spleen and kidney-Qi， the control group.Conclusion