抗AT1及α1受体自身抗体及高血压病合并肾损害关系.doc

抗AT1及α1受体自身抗体及高血压病合并肾损害关系 　作者：赵林双，廖玉华，王敏，周子华，向光大，候洁，乐岭，徐琳 【关键词】 抗AT1 　　Relationship between autoantibodies against AT1 and α1 receptors and hypertension with renal failure 　　　【Abstract】 AIM: To study the role of the autoantibodies against angiotensin Ⅱtype 1 receptor (AT1 receptor) and α1adrenergic receptor（α1 receptor） in the development of hypertension combined with renal failure. METHODS: The epitopes of the second extracellular loop of AT1 receptor (165-191) and α1 receptor (192-218) were synthesized and used respectively to screen sera autoantibodies from patients with hypertension and renal failure (n=61), patients with hypertension but without renal failure (n=60) and healthy blood donors (n=40, control) by ELISA and to screen the hormones of nerve and endocrine by RIA and by FIA. RESULTS: The positive rates of the autoantibodies against AT1 receptor (59.0%)and α1 receptor (54.1%) in patients with hypertension and renal failure were higher than those in patients with hypertension but without renal failure (13.3% and 10.0% respectively) (Plt;0.01) and they were also higher than those of healthy donors (10.0% and 7.5% respectively) (Plt;0.01). The hormones of nerve and endocrine (PRA, AngⅡ, ALD, E, NE) in patients with hypertension and renal failure were higher than those of patients with hypertension but without renal failure and they were also higher than those in healthy donors (Plt;0.05, Plt;0.01). CONCLUSION: Autoantibodies against AT1 receptor and α1 receptor may play some important roles in the pathogenesis of the hypertension combined with renal failure and activate accordingly the hormones of nerve and endocrine system in patients with the disease. 　　【Keywords】 hypertension/complication; kidney failure; receptors; autoantibodies 　　【摘要】 目的： 探讨抗血管紧张素Ⅱ受体1型（AT1受体）和α1肾上腺素受体（α1受体）自身抗体是否与高血压病合并肾损害有关. 方法：以合成的AT1和α1受体多肽片段为抗原，应用酶联免疫吸附测定（ELISA）技术，检测61例高血压病合并肾损害患者和60例高血压病无肾损害患者及40例正常人，血清中抗G蛋白偶联型AT1受体(165~191),和α1受体(192~218)自身抗体. 用放射免疫法,测定血浆肾素活性（PRA）、血管紧张素Ⅱ（AngⅡ）和醛