款冬花及其所含生物碱对小鼠肝脏毒性作用探究.docVIP

款冬花及其所含生物碱对小鼠肝脏毒性作用探究 　【摘要】 　　目的探讨款冬花提取物、总生物碱部位、非生物碱部位和克氏千里光碱（senkirkine）对小鼠肝脏的毒性作用。方法小鼠灌胃给予款冬花水提液、醇提液、总生物碱、非生物碱、克氏千里光碱及腹腔注射克氏千里光碱，连续给药7 d后，测定体重变化、肝脏指数，血清中GOT，GPT，TBIL的含量，并进行肝脏病理组织学检查。结果总碱组、克氏千里光碱口服和腹腔注射组显著抑制小鼠体重增加，血清生化指标显著升高，克氏千里光碱两组的肝脏指数明显增加。病理切片显示，水提组与对照组形态学无差异；总碱组形态学有所改变，肝细胞呈轻度细胞水肿，可见肝细胞点状坏死，肝小叶及汇管区未见炎细胞浸润；克氏千里光碱两组肝细胞均呈不同程度细胞水肿，可见肝细胞点状和碎片状坏死，肝小叶及汇管区可见少量炎细胞浸润。结论在所给剂量下，水提液无肝脏毒性，用药安全；总生物碱、克氏千里光碱有明显的肝脏毒性。 【关键词】 款冬花 肝脏毒性 小鼠 血清生化指标 病理组织学 　　Abstract：ObjectiveTo study the hepatotoxicity of the water and ethanol extracts, the total alkaloid and non-alkaloid parts of Flos Farfarae, as well as senkirkine, a pyrrolizidine alkaloid isolated from Flos Farfarae in mice. MethodsMice were given the aqueous and alcoholic extracts oraly, the total alkaloid and non-alkaloid parts, senkirkine (senkirkine-IG), respectively, and also intraperitoneal given senkirkine (senkirkine-IP), for 7 days. Hepatic injury effects were measured by experimental data of body weight, coefficient of liver, levels of GOT, GPT and TBIL in serum of mouse; histological examination of mouse liver was also carried out. ResultsThe total alkaloids, senkirkine IG or IP significantly inhibited the weight gain and increased the levels of GOT, GPT and TBIL in serum of mice; the senkirkine-IG and IP obviously increased the coefficient of liver. Compared with the control group, the results of histopathologic examination of the mouse liver showed that the livers of mice took the aqueous extract were not injured, the ones took the total alkaloids were slightly injured, and the ones took senkirkine (IG and IP) were significantly injured. ConclusionThe aqueous extract of Flos Farfarae is not toxic to liver of mice at the experimental dose, but the total alkaloids and senkirkine are toxic to liver of mice. 　　Key words：Flos Farfarae; Hepatotoxicity; Mice; Serum biochemical indicators; Histopathologic examination 　　款冬花为菊科植物款冬Tussilago farfara L.的干燥花蕾，又称冬花，性温、味辛，具有润肺下气、止咳化痰之功效，临床上主要用于新久咳嗽、喘咳痰多、劳嗽咳血等症［1］，为止咳平喘方剂中的常用中药。但是，化学