沙鼠短暂性脑缺血及西比灵干预后脑内Smad2及Smad4表达探究.docVIP

沙鼠短暂性脑缺血及西比灵干预后脑内Smad2及Smad4表达探究 　【摘要】 目的 研究沙鼠短暂性脑缺血及西比灵干预后脑内Smad2和Smad4蛋白的表达。方法 制作沙鼠脑缺血再灌注模型，用免疫组化法检测脑内Smad2、Smad4蛋白的表达变化。结果 各组沙鼠神经元和胶质细胞胞浆内均有Smad2、Smad4蛋白阳性表达，且Smad4蛋白表达均显著强于Smad2蛋白。缺血再灌注6h、1天后Smad2、Smad4蛋白表达显著上调(Plt;0.05)。同脑缺血组相比，西比灵干预组20只沙鼠神经元和神经胶质细胞内Smad2和Smad4蛋白在缺血再灌注6h、1天时表达明显下调(Plt;0.05)。结论 沙鼠脑神经细胞和神经胶质细胞内均有Smad2、Smad4蛋白表达；西比灵干预后，缺血再灌注沙鼠脑神经细胞和神经胶质细胞内Smad2和Smad4蛋白表达下调。提示Smad2和Smad4蛋白可能是缺血性脑损伤程度的又一重要标志；Smad4、Smad2在沙鼠脑缺血的发生和发展过程中可能有协同作用。 【关键词】 Smad蛋白；免疫组化；缺血再灌注；沙鼠 【Abstract】 Objective To study the expressions of Smad2 and Smad4 protein of flunarizine on the expressions of tissue following cerebral ischemic reperfusion in gerbil.Methods A cerebral ischemia - reperfusion model in gerbils was established . The expressions of Smad2 and Smad4 protein in brain tissue were detected by using immunohistochemistry technique at the 6th hour, 1st day after ischemic reperfusion (IR).Results Experimental results revealed that Smad2 and Smad4 protein were expressed in neurons and gliacytes of brain tissue in all gerbils, and expression of Smad4 protein is higher than that of Smad2 protein(Plt;0.05). Compared with normal control group, the expression of Smad2 and Smad4 protein in neurons and gliacytes of ischemic gerbils was evidently increased at the 6th hour, 1 day (Plt; 0.05).Compared with cerebral ischemia group, the expression of Smad2 and Smad4 protein in neurons and gliacytes of gerbils in flunarizine treatment group was evidently decreased at the 6th hour,1st day(Plt;0.05).Conclusion Smad2 and Smad4 protein were expressed in neurons and gliacytes of brain tissue in gerbils; and the expression of Smad2 and Smad4 protein in neurons and gliacytes of gerbils in flunarizine treatment group were evidently decreased.Smad2 and Smad4 protein might be important factors of mark of brain injury following cerebral ischemic reperfusion.Smad2 and Smad4 protein may take on synergistic actions in normal brain tissue of gerbils and in the occurrence and development of signal transduct