清肺化痰汤合红霉素对 COPD 痰热阻肺证大鼠模型疗效及机理探析.docVIP

清肺化痰汤合红霉素对 COPD 痰热阻肺证大鼠模型疗效及机理探析 　 作者：黄美杏 龚享文 陈斯宁 姚玉兰 张华玲 【摘要】 目的：探讨清肺化痰汤合红霉素对慢性阻塞性肺疾病（COPD）大鼠血清、肺泡灌洗液（BALF）中白介素-8（IL-8）表达及气道炎症的影响。方法：将 40 只 SPF 级 Wistar 大鼠随机分为 4 组，采用复合因素造模法建立COPD 大鼠模型，以观察清肺化痰汤合红霉素对 COPD 大鼠血清、BALF 中 IL-8 表达及气道炎症的影响。结果：与空白组比较，模型组各大鼠小气道出现组织细胞损伤、管壁炎症细胞浸润、血管增生及肺泡的融合，血清及 BALF 的 IL-8 的含量明显上升；与模型组比较，红霉素组在应用红霉素干预后大鼠小气道出现组织细胞损伤、管壁炎症细胞浸润、血管增生及肺泡的融合有所好转，血清及 BALF 的 IL-8 的含量明显下降，具有统计学意义；红霉素+清肺化痰汤组较红霉素组在同样的检测指标有明显下降。结论：清肺化痰汤合红霉素可以降低血及 BALF 中 IL-8 的表达，明显的抑制气道炎症的改变，提示清肺化痰汤具有治疗 COPD 气道炎症的作用。 【关键词】 清肺化痰汤；COPD；IL-8；气道炎症 〔Abstract〕 Objective To discuss the effects of Qingfei Huatan decoction combined with erythromycinin COPD (chronic obstructive pulmonary disease) model rats, including IL-8 in serum、BALF and airway inflammation. Methods 40 SPF level Wistar rats were randomly divided into 4 groups, COPD model rats were induced by complex factors, which is in order to observe the effects of Qingfei Huatan decoction combined with erythromycin on the expression of IL-8 in serum、BALF and airway inflammation. Results Compared with control group, model group appeared cell injury in small airway, inflammatory wall cells infiltration, angiogenesis and integration of alveoli, IL-8 levels in serum and BALF increased significantly; compared with those of model group, after the intervention, small airway tissue injury, wall infiltration of inflammatory cells, angiogenesis and integration of alveoli were all improved, IL-8 levels in serum and BALF decreased significantly in erythromycin group; compared with those of erythromycin group, indices of erythromycin + Qingfei Huatan decoction group were decreased significantly. Conclusion Qingfei Huatan decoction combined erythromycin can reduce the expression of IL-8 in serum and BALF, significantly inhibit the change of airway inflammation, suggesting Qingfei Huatan decoction can treat COPD airway inflammation. 〔Key Words〕 Qingfei Huatan decoction; COPD; IL-18; Airway inflammation 1 材料与方法 1.1 实验材料与仪器　 健康雄性 SPF 级 Wistar 大鼠，体重为（250±